CTHRC1在甲状腺乳头状癌组织中的表达及与E-cadherin和Vimentin表达的相关性分析

唐振宁; 王玉琛; 刘晓霞; 刘奇伦

doi:10.13201/j.issn.1001-1781.2018.08.009

CTHRC1在甲状腺乳头状癌组织中的表达及与E-cadherin和Vimentin表达的相关性分析

- 1. 宁夏医科大学总医院肿瘤外三科(银川, 750004);
- 2. 宁夏银川市第一人民医院病理科
基金项目:
宁夏回族自治区卫生厅重点科研计划课题 (No:2013104)

详细信息

通讯作者: 刘奇伦, E-mail:liuql6311@126.com

中图分类号: R736.1

收稿日期: 2017-12-17

An immunohistochemical study of CTHRC1,Vimentin,E-cadherin expression in papillary thyroid carcinoma

- 1 Department of Oncology Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, China;
- 2 Department of Pathology, the First People's Hospital of Yinchuan

More Information

Corresponding author: LIU Qilun, E-mail: liuql6311@126.com

Received Date: 17 December 2017

摘要

摘要: 目的: 探讨甲状腺乳头状癌组织中CTHRC1的表达及其与肿瘤发生发展的关系。方法: 选取甲状腺乳头状癌组织63例与相应癌旁正常甲状腺组织36例以及结节性甲状腺肿组织23例,使用免疫组织化学方法检测CTHRC1蛋白的表达,分析CHTRC1表达与甲状腺乳头状癌患者临床病理特征的关系及其与E-cadherin、Vimentin表达的相关性。结果: CTHRC1蛋白在甲状腺乳头状癌组织中的表达水平明显高于癌旁正常组织和良性病变组织(P<0.01);CTHRC1在甲状腺乳头状癌淋巴结转移患者中的表达水平明显增高(P<0.05)。甲状腺乳头状癌组织中CTHRC1和E-cadherin、Vimentin的表达相关(均P<0.01)。结论: CTHRC1与甲状腺乳头状癌的发生、发展相关,可能参与了甲状腺乳头状癌上皮-间质转化过程。
- 甲状腺肿瘤 /
- CTHRC1 /
- 上皮-间质转化 /
- 免疫组织化学
Abstract: Objective: To investigate the expression and significance of CTHRC1 in patients with papillary thyroid cancinoma.Method: We collected 63 specimens of papillary thyroid carcinoma tissue with 36 specimens of the adjacent normal thyroid tissue and 23 specimens of thyroid nodular goiter. The expressions of CTHRC1 protein in these tissues were detected by immunohistochemical staining. The correlations between the expressions of CTHRC1 with clinicopathologic features, E-cadherin and Vimentin expression were analyzed.Result: CTHRC1 protein expression levels in papillary thyroid carcinoma tissue were significantly higher than those in adjacent normal thyroid tissue and benign disease (P<0.01); CTHRC1 expression was significantly correlated with lymph node metastases(P<0.05). Moreover, CTHRC1 expression was correlated with the expression of E-cadherin and Vimentin (P<0.01).Conclusion: CTHRC1 is related with the occurrence and miligant transformation of papillary thyroid cancinoma. We speculated that CTHRC1 might play a role in the epithelial-mesenchymal transition of papillary thyroid cancinoma.
- thyroid neoplasms /
- collagen triple helix repeat containing 1 /
- epithelial-mesenchymal transition /
- immunohistochemistry

HTML全文

参考文献(21)

[1]	LA VECCHIA C, MALVEZZI M, BOSETTI C, et al.Thyroid cancer mortality and incidence:aglobal overview[J].Int J Cancer, 2015, 136:2187-2195.
[2]	孙琳, 王冬梅, 张文静, 等.血清E-cadherin在甲状腺乳头状癌中的表达及意义[J].临床耳鼻咽喉头颈外科杂志, 2017, 31 (2):123-126.
[3]	田文.分化型甲状腺癌初始手术治疗的争论焦点[J].中国普通外科杂志, 2015, 24 (11):1501-1503.
[4]	LEE J, SONG J, KWON E S, et al.CTHRC1 promotes angiogenesis by recruiting Tie2-expressing monocytes to pancreatic tumors[J].Exp Mol Med, 2016, 48:e261.
[5]	ERIKSSON J, LE JONCOUR V, NUMMELA P, et al.Gene expression analyses of primary melanomas reveal CTHRC1 as an important player in melanoma progression[J].Oncotarget, 2016, 7:15065-15092.
[6]	ZHANG R, CAO Y, BAI L, et al.The collagen triple helix repeat containing 1facilitates hepatitis B virus-associated hepatocellular carcinoma progression by regulating multiple cellular factors and signal cascades[J].Mol Carcinog, 2015, 54:1554-1566.
[7]	YAN L, YU J, TAN F, et al.SP1-mediated microR-NA-520d-5p suppresses tumor growth and metastasis in colorectal cancer by targeting CTHRC1[J].Am JCancer Res, 2015, 5:1447-1459.
[8]	CHEN S, LI P, YANG R, et al.microRNA-30binhibits cell invasion and migration through targeting collagen triple helix repeat containing 1in non-small cell lung cancer[J].Cancer Cell Int, 2015, 15:85.
[9]	YU J, FENG J, ZHI X, et al.Let-7binhibits cell proliferation, migration, and invasion through targeting Cthrc1 in gastric cancer[J].Tumour Biol, 2015, 36:3221-3229.
[10]	HOU M, CHENG Z, SHEN H, et al.High expression of CTHRC1 promotes EMT of epithelial ovarian cancer (EOC) and is associated with poor prognosis[J].Oncotarget, 2015, 6:35813-35829.
[11]	LIU W, FU X L, YANG J Y, et al.Elevated expression of CTHRC1 predicts unfavorable prognosis in patients with pancreatic ductal adenocarcinoma[J].Am J Cancer Res, 2016, 6:1820-1827.
[12]	SANG W, ZHU L, MA J, et al.Lentivirus-Mediated Knockdown of CTHRC1 Inhibits Osteosarcoma Cell Proliferation and Migration[J].Cancer Biother Radiopharm, 2016, 31:91-98.
[13]	LEE C E, VINCENT-CHONG V K, RAMANATHANA, et al.Collagen Triple Helix Repeat Containing-1 (CTHRC1) Expression in Oral Squamous Cell Carcinoma (OSCC):Prognostic Value and Clinico-Pathological Implications[J].Int J Med Sci, 2015, 12:937-945.
[14]	TANG L, DAI D L, SU M, et al.Aberrant expression of collagen triple helix repeat containing 1in human solid cancers[J].Clin Cancer Res, 2006, 12:3716-3722.
[15]	LIU G, WU K, SHENG Y.Elucidation of the molecular mechanisms of anaplastic thyroid carcinoma by integrated miRNA and mRNA analysis[J].Oncol Rep, 2016, 36:3005-3013.
[16]	WANG S F, YIN Z, YIN J J, et al.Clinical significance of CTHRC1 protein expression in human cancers:a meta-analysis[J].Genet Mol Res, 2016, 15 (2).
[17]	李晓京, 杨乐, 马斌林.1988例甲状腺微小乳头状癌颈部中央区淋巴结转移危险因素分析[J].临床耳鼻咽喉头颈外科杂志, 2017, 31 (1):52-57.
[18]	LIANG Z, XIE W J, ZHAO M, et al.DDR2 facilitates papillary thyroid carcinoma epithelial mesenchymal transition by activating ERK2/Snail1 pathway[J].Oncol Lett, 2017, 14:8114-8121.
[19]	WANG Y, LEE M, YU G, et al.CTHRC1 activates pro-tumorigenic signaling pathways in hepatocellular carcinoma[J].Oncotarget, 2017, 8:105238-105250.
[20]	LIU J, LI W, LIU S, et al.Knockdown of Collagen Triple Helix Repeat Containing 1 (CTHRC1) Inhibits Epithelial-Mesenchymal Transition and Cellular Migration in Glioblastoma Cells[J].Oncol Res, 2017, 25:225-232.
[21]	LAMOUILLE S, XU J, DERYNCK R.Molecular mechanisms of epithelial-mesenchymal transition[J].Nat Rev Mol Cell Biol, 2014, 15:178-196.