变应性鼻炎中自噬相关基因LC3与ECP的表达及意义

余杰情; 罗庆; 熊园平; 康雪; 罗丽萍; 张剑; 黄小燕; 叶菁

doi:10.13201/j.issn.1001-1781.2019.04.009

变应性鼻炎中自噬相关基因LC3与ECP的表达及意义

- 南昌大学第一附属医院耳鼻咽喉头颈外科(南昌, 330006)
基金项目:
江西省科技厅青年科学基金资助项目(No:20171BAB215035)

江西省教育厅青年项目(No:GJJ170130)

江西省卫计委课题(No:20195123)

详细信息

通讯作者: 叶菁,E-mail:1292446109@qq.com

中图分类号: R765.21

收稿日期: 2019-01-28

Expression of LC3 and ECP in allergic rhinitis and their significance

- Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Nanchang University, Nanchang, 330006, China

More Information

Corresponding author: YE Jing, E-mail: 1292446109@qq.com

Received Date: 28 January 2019

摘要

摘要: 目的:探讨自噬相关基因微管相关蛋白1轻链3(LC3)在变应性鼻炎(AR)患者鼻黏膜中的表达及其与嗜酸粒细胞阳离子蛋白(ECP)的关系。方法:选取20例AR患者下鼻甲黏膜(AR组)和20例正常鼻腔黏膜(对照组),苏木精-伊红染色观察各组鼻黏膜的中嗜酸粒细胞的分布与表达,采用免疫组织化学方法、蛋白免疫印迹法检测各组鼻黏膜中LC3和ECP的表达情况。结果:苏木精-伊红染色法显示在AR组患者鼻腔黏膜中可观察到大量嗜酸粒细胞及淋巴细胞浸润。免疫组织化学结果显示LC3与ECP主要分布于AR鼻黏膜上皮内,较对照组明显增多,差异有统计学意义(P<0.05)。蛋白免疫印迹法示LC3和ECP在AR组中的表达较对照组显著上升(P<0.05),AR组中LC3与ECP的表达呈正相关(P<0.05)。结论:LC3和ECP在AR患者中表达升高且具有相关性,提示自噬在AR的毒性作用中起了重要作用,可为AR的精准化治疗提供理论依据。
- 鼻炎 /
- 变应性 /
- 微管相关蛋白1轻链3 /
- 嗜酸粒细胞阳离子蛋白
Abstract: Objective: To investigate the expression of microtubule-associated protein 1 light chain 3 beta(LC3) and eosinophil cationic protein in allergic rhinitis(AR) for further understanding of the pathogenesis of AR. Method: Twenty cases of normal nasal mucosa and 20 cases of AR nasal mucosa were collected. Histological changes of nasal mucosa were examined by hematoxylin and eosin(HE) staining. The expression of LC3 and ECP were measured by immunohistochemistry(IHC) and Western Blot(WB). Result: The tissue samples demonstrated a large number of eosinophils and lymphocytes infiltration in AR. IHC revealed that LC3 and ECP expression were higher in AR than in normal nasal mucosa(P<0.05). WB also showed that the relative expression levels of protein expression of LC3 and ECP were greater in AR than in controls. The expression level of LC3 was positively correlated with that of ECP protein in AR.Conclusion: LC3 and ECP were upregulated and positively correlated in AR, indicating that autophagy plays an important role in the toxicity of allergic rhinitis, which provides theoretical basis for the precise treatment of AR.
- rhinitis /
- allergic /
- microtubule-associated protein 1 light chain 3 beta /
- eosinophil cationic protein

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