Determination of pathological margin of hypopharyngeal cancer by terahertz time-domain spectroscopy system
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摘要: 目的 探讨太赫兹系统鉴别下咽癌肿瘤组织与正常组织的准确性, 及其在下咽癌病理切缘判断中的作用。方法 选用SPF级5周龄雄性BALB/c裸鼠, 皮下注射法构建人下咽癌裸鼠移植瘤模型。将获得的移植瘤标本进行病理学诊断, 以确定肿瘤组织范围。利用太赫兹时域光谱系统分别扫描人下咽癌裸鼠移植瘤肿瘤组织、正常组织和癌旁组织标本。通过对冷冻切片和石蜡切片的检测, 获得差异性光谱数据, 将所得数据进行t检验比较。结果 肿瘤组织的太赫兹吸收系数高于正常组织, 冷冻切片中当检测频率大于0.48 THz时其差异有统计学意义(P < 0.05);石蜡切片中在0.2~1.6 THz的有效频谱范围内, 其差异有统计学意义(P < 0.01)。经两种方式处理的标本, 肿瘤组织和正常组织折射率差异均有统计学意义(P < 0.01)。癌旁组织太赫兹吸收系数落在正常组织和肿瘤组织之间。结论 太赫兹技术能够准确判断肿瘤组织和正常组织, 并且能够灵敏地检测出癌旁组织的光谱变化, 将可能成为病理切缘诊断的另一种工具。Abstract: Objective To investigate the accuracy of the terahertz system in differentiating hypopharyngeal cancer from normal tissue and its role in determining the pathological incised margin of hypopharyngeal cancer.Method The transplantation model of hypopharyngeal cancer in 5-week-old male BALB/c nude mice were established by subcutaneous injection. The obtained transplanted tumor specimens were pathologically diagnosed to determine the extent of tumor tissue. Tumor tissue, normal tissue and paracellular tissue of transplantation tumor were scanned by terahertz time-domain spectroscopy. The differential spectral data were obtained through the detection of frozen tissue sections and paraffin tissue sections, respectively. The results were compared by t-test.Result The tarahertz absorption coefficient of tumor tissues was higher than that of normal tissues, and the difference was statistically significant when the detection frequency was greater than 0.48 THz in frozen sections(P < 0.05). In the paraffin sections, the difference was statistically significant within the effective spectrum range of 0.2-1.6 THz(P < 0.01). The difference of refractive index between tumor tissue and normal tissue was significant in specimens treated in two ways(P < 0.01). The absorption coefficient of adjacent tissue felt between that of normal tissue and tumor tissue.Conclusion Terahertz technology can distinguish tumor tissues and normal tissues accurately, and can detect the spectral changes in adjacent tissues sensitively. Therefore, terahertz technology may become another tool for the judgment of pathologic margin.
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