Abstract: Objective: To analyze the pathogenic bacteria distribution and drug resistance of secretion in 90 child patients with acute otitis media. Method: The clinical data of 90 cases of acute otitis media child patients were retrospectively analyzed. The distribution of pathogenic bacteria in all child patients were analyzed. The child patients were divided into A group(less than 1 year old, n=38), B group(1 to 3 years old, n=27) and C group(more than 3 years old, n=25) according to the age. The streptococcus pneumoniae detection in each group and the streptococcus pneumoniae resistant to various antibacterial agents were compared. Result: The positive rate of pathogen detection in children was 75.6%(68/90), and the highest proportion was streptococcus pneumoniae with 61.8%(42/68). The detection rate of streptococcus pneumoniae in A and B group was significantly higher than that of C group(P<0.05). The resistance rate of streptococcus pneumoniae to different antibacterial drugs is quite different.Conclusion: Streptococcus pneumoniae is the main pathogenic bacterium in child patients with acute otitis media less than 3 years old, and different types of antibacterial drugs have different drug resistance.