rmIL-33诱导的nuocyte细胞促进变应性鼻炎小鼠模型的炎症反应

蔺林; 戴飞; 魏瑾瑾; 陈峥; 汤欣玥

doi:10.13201/j.issn.1001-1781.2019.09.018

rmIL-33诱导的nuocyte细胞促进变应性鼻炎小鼠模型的炎症反应

- 复旦大学附属华山医院北院耳鼻咽喉头颈外科(上海, 200040)
基金项目:
国家自然科学基金(No:81371076)

上海申康医院发展中心郊区医院临床能力建设项目(No:SHDC12015905)

复旦大学附属华山医院北院启动基金(No:HSBY2016005)

详细信息

通讯作者: 陈峥,E-mail:wallwallsd1111@163.com

中图分类号: R765.21

收稿日期: 2018-12-21

rmIL-33-stimulated nuocytes promote allergic inflammation in mouse model of allergic rhinitis

- Department of Otorhinolaryngology Head and Neck Surgery, Huashan Hospital North of Fudan University, Shanghai, 200040, China

More Information

Corresponding author: CHEN Zheng, E-mail: wallwallsd1111@163.com

Received Date: 21 December 2018

摘要

摘要: 目的:探讨nuocyte细胞对变应性鼻炎(AR)小鼠模型的作用。方法:将人重组(rm)白细胞介素(IL)-33滴入BALB/c小鼠鼻腔,分离、提纯并体外培养小鼠鼻相关淋巴组织(NALT)中的nuocyte细胞,体外检测该细胞对rmIL-33干预后的反应;用卵清蛋白建立AR小鼠模型,将体外培养的NALT来源的nuocyte细胞过继转移给AR小鼠,检测其变应性炎症反应。结果:rmIL-33干预后小鼠NALT中nuocyte细胞数目明显多于AR组小鼠(t=3.66,P<0.01);体外培养的nuocyte细胞经rmIL-33干预后释放IL-13的浓度显著高于未干预组(t=19.90,P<0.000 1);nuocyte细胞过继转移后,其打喷嚏次数(t=9.89,P<0.000 1)、鼻腔冲洗液中嗜酸粒细胞数目(t=8.17,P<0.000 1)以及IL-13(t=40.47,P<0.000 1)和IL-33的浓度(t=19.89,P<0.000 1)均高于AR小鼠。结论:nuocyte细胞促进了小鼠AR模型的炎症反应。
- nuocyte细胞 /
- 鼻炎,变应性 /
- 小鼠 /
- 鼻相关淋巴组织 /
- 白细胞介素
Abstract: Objective: The study aimed to investigate the role of nuocytes in allergic rhinitis (AR) murine models. Method: After intranasal administration of recombinant (rm) interleukin (IL)-33 in BALB/c mice, nuocytes were sorted and purified from the mouse nasal-associated lymphoid tissue (NALT). Then, we examined the response of nuocytes to rmIL-33 in vitro. After a murine model of AR was established using ovalbumin, we adoptively transferred the cultured NALT-derived nuocytes to mice models, and determined allergic responses in them. Result: rmIL-33 expanded nuocytes in NALT of mice compared with AR mice (t=3.66, P<0.01), and increased production of IL-13 from these cells in vitro in comparison with unstimulated nuocytes (t=19.90, P<0.000 1). After adoptive transfer of nuocytes, sneezing (t=9.89, P<0.000 1) ,numbers of eosinophils(t=8.17, P<0.000 1), concentrations of IL-13 (t=40.47, P<0.000 1) and IL-33 (t=19.89, P<0.000 1) in nasal lavage fluid were all enhanced when compared with AR mice. Conclusion: Nuocytes promote allergic inflammation in a murine model of AR.
- nuocytes /
- rhinitis,allergic /
- mouse /
- nasal-associated lymphoid tissue /
- interleukin

HTML全文

参考文献(18)

[1]	PAUL W E,ZHU J.How are T(H)2-type immune responses initiated and amplified[J]? Nat Rev Immunol,2010,10:225-235.
[2]	MATSUSHITA K,KATO Y,AKASAKI S,et al.Proallergic cytokines and group 2 innate lymphoid cells in allergic nasal diseases[J].Allergol Int,2015,64:235-240.
[3]	NEILL D R,WONG S H,BELLOSI A,et al.Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity[J].Nature,2010,464:1367-1370.
[4]	BARLOW J L,BELLOSI A,HARDMAN C S,et al.Innate IL-13-producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity[J].J Allergy Clin Immunol,2012,129:191-198.e1-4.
[5]	GHAFFAR O,LABERGE S,JACOBSON M R,et al.IL-13 mRNA and immunoreactivity in allergen-induced rhinitis:comparison with IL-4 expression and modulation by topical glucocorticoid therapy[J].Am J Respir Cell Mol Biol,1997,17:17-24.
[6]	MIYAHARA S,MIYAHARA N,MATSUBARA S,et al.Physiological assessment of allergic rhinitis in mice:role of the high affinity IgE receptor(FceRI)[J].J Allergy Clin Immunol,2005,116:1020-1027.
[7]	MIYAHARA S,MIYAHARA N,MATSUBARA S,et al.IL-13 is essential to the late-phase response in allergic rhinitis[J].J Allergy Clin Immunol,2006,118:1110-1116.
[8]	LIN L,DAI F,WEI J J,et al.Allergic inflammation is exacerbated by allergen-induced type 2 innate lymphoid cells in a murine model of allergic rhinitis[J].Rhinology,2017,55:339-347.
[9]	HIROMURA Y,KISHIDA T,NAKANO H,et al.IL-21 administration into the nostril alleviates murine allergic rhinitis[J].J Immunol,2007,179:7157-7165.
[10]	COSTA M F,BORNSTEIN V U,CANDÉA A L,et al.CCL25 induces α₄β₇ integrin-dependent migration of IL-17+γδ T lymphocytes during an allergic reaction[J].Eur J Immunol,2012,42:1250-1260.
[11]	PAUL W E.What determines Th2 differentiation,in vitro and in vivo[J]? Immunol Cell Biol,2010,88:236-239.
[12]	WALTER D M,MCINTIRE J J,BERRY G,et al.Critical role for IL-13 in the development of allergen-induced airway hyperreactivity[J].J Immunol,2001,167:4668-4675.
[13]	SPITS H,ARTIS D,COLONNA M,et al.Innate lymphoid cells--a proposal for uniform nomenclature[J].Nat Rev Immunol,2013,13:145-149.
[14]	FAHY J V,LOCKSLEY R M.The airway epithelium as a regulator of Th2 responses in asthma[J].Am J Respir Crit Care Med,2011,184:390-392.
[15]	MJÖSBERG J M,TRIFARI S,CRELLIN N K,et al.Human IL-25-and IL-33-responsive type 2 innate lymphoid cells are defined by expression of CRTH2 and CD161[J].Nat Immunol,2011,12:1055-1062.
[16]	FORT M M,CHEUNG J,YEN D,et al.IL-25 induces IL-4,IL-5,and IL-13 and Th2-associated pathologies in vivo[J].Immunity,2001,15:985-995.
[17]	SCHMITZ J,OWYANG A,OLDHAM E,et al.IL-33,an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines[J].Immunity,2005,23:479-490.
[18]	张强,徐佳,李玉茹,等.变应性鼻炎的细胞因子免疫机制进展[J].临床耳鼻咽喉头颈外科杂志,2012,26(23):1102-1104.