nuocyte细胞体外诱导变应性鼻炎小鼠初始T细胞向Th2细胞的分化

蔺林; 戴飞; 魏瑾瑾; 陈峥; 汤欣玥

doi:10.13201/j.issn.1001-1781.2018.20.010

nuocyte细胞体外诱导变应性鼻炎小鼠初始T细胞向Th2细胞的分化

- 复旦大学附属华山医院耳鼻咽喉头颈外科(上海, 200040)
基金项目:
国家自然科学基金 (No:81371076)

上海申康医院发展中心郊区医院临床能力建设项目 (No:SHDC12015905)

详细信息

通讯作者: 蔺林, E-mail:linlinhsn@aliyun.com

中图分类号: R765.21

收稿日期: 2018-08-26

Differentiation of naive T cells into Th2 cells induced by nuocyte cells in mice with allergic rhinitis

- Department of Otorhinolaryngology Head and Neck Surgery,Huashan Hospital of Fudan University, Shanghai,200040, China

More Information

Corresponding author: LIN Lin, E-mail: linlinhsn@aliyun.com

Received Date: 26 August 2018

摘要

摘要: [HT4"SS]
目的: 探讨nuocyte细胞是否可以在体外诱导AR小鼠的初始T细胞向Th2细胞分化。方法: 用卵清蛋白建立变应性鼻炎(AR)小鼠模型,分离、提纯并体外培养小鼠鼻相关淋巴组织(NALT)中的nuocyte细胞,检测该细胞是否可以合成IL-4,获取AR小鼠外周血单个核细胞并分离出Th2细胞和T细胞进行体外培养,检测其中Th2细胞占T细胞百分比,然后将体外培养的NALT来源的nuocyte细胞加入上述小鼠T细胞培养液共培养,再次检测Th2细胞百分比。结果: AR小鼠模型的打喷嚏次数、挠鼻次数及鼻腔冲洗液中嗜酸粒细胞数均高于正常小鼠,以流式细胞仪技术将NLAT中nuocyte细胞鉴定为CD3CD4CD8CD19CD11bCD11cFcεR1(lineage)^-ICOS⁺,该细胞表达IL-4,而且AR小鼠nuocyte细胞中IL-4蛋白和mRNA含量均高于正常小鼠,nuocyte细胞与T细胞共培养后,Th2细胞所占T细胞百分比明显升高。结论: nuocyte细胞可能通过IL-4通路在体外诱导AR小鼠初始T细胞向Th2细胞分化。
- nuocyte细胞 /
- 鼻炎 /
- 变应性 /
- 小鼠 /
- 鼻相关淋巴组织 /
- 白细胞介素
Abstract: Objective: The aim of this study is to examine whether nuocytes induced differentiation of primary T cells into Th2 cells in AR mice in vitro.Method: A murine model of AR on the background of BALB/c was established using ovalbumin, and nuocytes were sorted and purified from the mouse nasal associated lymphoid tissue (NALT) and cultured in vitro. Then, we assessed the expression of IL-4 in these cells. Mouse peripheral blood mononuclear cells were isolated and Th2 cells and T cells were isolated for in vitro culture, and the percentage of T cells in Th2 cells was detected. Then, NALT-derived nuocyte cells cultured in vitro were added to the above-mentioned mouse T cell culture medium for co-culture.Result: Numbers of sneezing, nasal rubbing and eosinophils in nasal lavage fluid were all enhanced in AR mice compared to normal ones. We used flow cytometry analysis to identify nuocytes from mice NALT as CD3CD4CD8CD19CD11bCD11cFcεR1 (lineage)^-ICOS⁺, and also found that the cells expressed IL-4, and its protein and mRNA were all increased in AR mice versus normal mice. After nuocytes were co-cultured with T-cell cultures, we determined the percentage of Th2 cells in total T cells, and found that the percentage was increased significantly.Conclusion: nuocyte cells may induce the differentiation of primary T cells into Th2 cells in AR mice through IL-4 pathway.
- nuocytes /
- rhinitis /
- allergic /
- mouse /
- nasal-associated lymphoid tissue /
- interleukin

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