Gene expression profile analysis involved in pathogenesis of allergic rhinitis by using bioinformatics method as a novel way of drug screening
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摘要: 目的: 探讨变应性鼻炎(AR)发病相关基因表达谱特征,并根据药物与疾病基因表达谱相似性对比的原理,筛选出可能对AR具有治疗作用的潜在新型表观药物。方法: 以AR为关键词,在开放的人类基因表达数据库和基因芯片数据库中检索与AR发病相关的基因表达谱,并进行聚类分析。然后,在药物基因表达谱数据库中筛选与AR发病相关基因表达谱特征相反者。结果: 检索到1个满足条件的相关基因表达数据库GSE43523。与AR发病相关的基因共有11个(表达水平变化超过2倍),其中上调者7个,下调者4个。对AR发病相关基因谱与药物基因表达谱相似性分析发现,嘌呤霉素、脱氧前列腺素J2、泼尼松、苯海索、甲亢平、林可霉素、金鸡纳啶、可可碱、雷公藤和多西拉敏等10种药物都具有治疗AR的作用。结论: 采用生物信息学方法,对比疾病发病相关基因表达谱与药物基因表达谱的相似性,有可能成为AR治疗药物筛选的新方法。Abstract: Objective: In this study, the characteristics of gene expression profiles of allergic rhinitis (AR) were preliminarily explored. Based on the comparison of the similarity between drug and disease gene expression profiles, new drugs that could treat AR were screened out.Method: AR was used as the key word to search gene expression datasets related to pathogenesis of AR in public Gene Expression Omnibus (GEO) of NCBI. After significance test, gene expression profiles involved in pathogenesis of AR were screened and applied to cluster analysis. And then AR related gene expression profiles were thrown into pharmacological gene expression datasets for similarity analysis and significantly negative correlation was used as a screening criterion for potential curative drugs of AR.Result: Only one gene expression dataset was found out, ie GSE43523. Computational bioanalysis identified a total of 11 candidate genes involved in pathogenesis of AR, whose expression level exceeded more than 2-fold. Among them, 7 genes were upregulated and 4 genes were downregulated. Similarity analysis of gene expression profiles of AR showed that puromycin, 15-delta prostaglandin J2, prednisone, trihexyphenidyl, carbimazole, lincomycin, cinchonidine, theobromine, tripterygium wilfordii and doxylamine might have potential therapeutic effects on AR.Conclusion: It is concluded that by means of bioinformatics method, similarity analysis between disease related gene expression and pharmacological gene expression profiles may be a novel way of drug screening for AR.
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Key words:
- allergic rhinitis /
- bioinformatics /
- drug screening /
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