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摘要: 目的：建立动物实验VEMPs检测方法，确定正常豚鼠气导声刺激（ACS）的颈肌前庭诱发肌源性电位（cVEMP）、眼肌前庭诱发肌源性电位（oVEMP）的实验方法和波形引出特点，从而为cVEMP和oVEMP的后续研究奠定实验基础。方法：选择11只健康豚鼠（250~350 g），使用特制的动物固定架，保持动物俯卧抬头位，在清醒状态下行ACS-cVEMP、ACS-oVEMP，建立正常豚鼠的VEMPs测试方法并记录分析波形特点和参数结果。结果：11只（22耳）健康豚鼠均可引出气导声刺激诱发的ACS-cVEMP和ACS-oVEMP。ACS-cVEMP和ACS-oVEMP的引出率分别为77.27%和59.09%；阈值分别为（107.1±14.6）dB SPL和（115.5±15.6）dB SPL；cVEMP的n1、p1潜伏期分别为（7.4±1.5）ms和（9.3±1.6）ms，oVEMP的n1、p1潜伏期分别为（6.4±1.4）ms和（8.1±1.8）ms；振幅cVEMP和oVEMP分别为（15.4±8.6）μV和（11.4±6.0）μV。在阈值、n1潜伏期、p1潜伏期和振幅参数方面，cVEMP和oVEMP无统计学差异（P>0.05）。结论：通过本实验方法，成功在动物（豚鼠）身上诱发出稳定的气导声刺激诱发的VEMPs波形，包括cVEMP和oVEMP，并测得正常豚鼠VEMPs的参数值，包括引出率、阈值、潜伏期和振幅，为将来各种内耳疾病评估前庭功能的模型奠定了良好的实验基础。Abstract: Objective: To establish detection methods of air-conducted sound elicited vestibular-evoked myogenic potentials (ACS-VEMPs) in guinea pigs, including cervical vstibular-evoked myogenic potential (cVEMP) and ocular vestibular-evoked myogenic potential (oVEMP).Method: Eleven healthy (250-350 g) guinea pigs (22 ears) were selected and underwent conventional ACS-cVEMP and ACS-oVEMP examinations. Parameters of waveforms were recorded.Result: The ACS-cVEMP and ACS-oVEMP could be elicited in 77.27% and 59.09% guinea pigs, respectively;threshold was (107.1±14.6)dB SPL and (115.5±15.6)dB SPL for ACS-cVEMP and ACS-oVEMP;n1,p1 latency for cVEMP was (7.4±1.5)ms and (9.3±1.6)ms,(6.4±1.4)ms and (8.1±1.8)ms for oVEMP;amlipude was (15.4±8.6)μV and (11.4±6.0)μV for cVEMP and oVEMP respectively. No statistical difference could be found in above parameters between ACS-cVEMP and ACS-oVEMP (P>0.05).Conclusion: Both ACS-cVEMP and ACS-oVEMP could be elicited in guinea pigs.
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Key words:
- guinea pigs /
- vestibular evoked myogenic potentials /
- air-conducted sound
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