Expression of IL-25 and IL-33 and the count of EOS in peripheral blood of children with allergic rhinitis receiving immunotherapy
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摘要: 目的:探讨儿童变应性鼻炎(AR)患者外周血中IL-25与IL-33水平和EOS计数及其临床意义。方法:选取94例接受免疫治疗的儿童AR患者,23例健康人。采用酶联免疫(ELISA)检测入选者外周血IL-25和IL-33水平,检测EOS计数。结果:轻度组IL-25、IL-33水平高于对照组(P＜0.05);轻度组EOS计数与对照组EOS计数差异无统计学意义(P＞0.05);重度组IL-25、IL-33水平高于轻度组和对照组(均P＜0.05);重度组EOS计数高于轻度组和对照组(均P＜0.05)。Spearman检测AR患者IL-25水平与IL-33水平呈正相关(R=0.238,P＜0.05)。结论:IL-25、IL-33及EOS计数在AR患者中表达均增强,提示它们可能参与AR的发病过程。了解它们之间的作用机制,可能有助于治疗AR。Abstract: Objective:To investigate the expression of IL-25,IL-33 and EOS in children with allergic rhinitis (AR).Method:Ninety-four AR children receiving immunotherapy and 23 healthy people were concluded in the study. The serum levels of IL-25 and IL-33 were detected by Enzyme-linked Immunosorbent Assay (ELISA), and a count of EOS were measured.Result:The serum levels of IL-25 and IL-33 in the mild group were higher than control group (P＜0.05). The count of EOS showed no difference between the mild group and the control group (P＞0.05). The serum levels of IL-25 and IL-33 in the severe group were higher than those in mild group (P＜0.05). The serum levels of IL-25 and IL-33 in the severe group were higher than control group (P＜0.05). The count of EOS in the severe group were higher than those in mild group (P＜0.05). The count of EOS in the severe group were higher than those in control group (P＜0.05). Spearman test showed the serum levels of IL-25 in the children with AR patients have positive correlation with the serum levels of IL-33 (P＜0.05, R=0.238).Conclusion:Expression of IL-25 levels, IL-33 levels and the count of EOS in patients with AR are enhanced, which shows that IL-25, IL-33 and the count of EOS are involved in the AR. If we can understand the mechanism of them, it will profound implications for treatment.
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Key words:
- rhinitis /
- allergic /
- IL-25 /
- IL-33 /
- eosinophils
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