Effects of hyperbaric oxygen on mucosal inflammatory response after nasal surgery in rabbits
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摘要: 目的：观察高压氧治疗后兔鼻腔手术区炎症反应的情况，探讨高压氧治疗在鼻腔手术后恢复过程中降低炎性反应、促进黏膜愈合的临床可行性。方法：合格日本大耳白兔16只创建双侧鼻腔手术模型，随机分为高压氧组和非高压氧组各8只，另设5只正常对照组。非高压氧组术后处于常规常压环境，高压氧组术后第5天开始高压氧治疗。于术后第6周处死全部动物，取双侧下鼻甲手术区黏膜观察大体形态及病理学改变。正常对照组取相同部位黏膜观察。结果：大体标本：高压氧组术区积脓2侧，非高压氧组8侧，高压氧组较非高压氧组明显减少，差异有统计学意义（P<0.05）；病理改变：高压氧组炎细胞浸润轻度12侧，中度4侧；非高压氧组轻度1侧，中度13侧，重度2侧，高压氧组较非高压氧组明显减轻，差异有统计学意义（P<0.01）。结论：在本实验条件下，高压氧治疗可明显减轻兔鼻腔手术后黏膜的炎症反应，减少术后鼻腔脓性分泌物的积蓄。Abstract: Objective: To observe the effect of hyperbaric oxygen therapy for the inflammation of rabbit nasal in postoperation period, to investigate the clinical feasibility of hyperbaric oxygen therapy in reducing the inflammatory reaction and promoting mucosal healing in nasal recovery stage after surgery.Method: A total of 16 Japanese white rabbits were randomly assigned to hyperbaric oxygen group and non hyperbaric oxygen group, with 8 in each, and another 5 was divided into normal control group. The non hyperbaric oxygen group was in the normal atmospheric environment, the hyperbaric oxygen group was given hyperbaric oxygen treatment on the fifth day after the operation. All the animals were sacrificed to observe the morphological changes and pathological changes of the mucosa in the bilateral inferior turbinate surgery area at sixth weeks after operation. The normal control group was taken the same part of mucosa.Result: Specimen:hyperbaric oxygen group postoperative empyema in 2 side, 8 side of non hyperbaric oxygen group, hyperbaric oxygen group was significantly reduced compared with non hyperbaric oxygen group, the difference was statistically significant(P<0.05). Pathological changes:in the hyperbaric oxygen group, the infiltration of inflammatory cells was mild in 12 sides and moderate in 4 sides; the non hyperbaric oxygen group was mild in 1 sides, moderate in 13 sides, and severe in 2 sides, the hyperbaric oxygen group was significantly lower than the non hyperbaric oxygen group, the difference was statistically significant(P<0.01).Conclusion: Under the condition of this experiment, hyperbaric oxygen therapy can significantly reduce the inflammatory response of rabbit nasal mucosa after operation, and reduce the accumulation of purulent secretion.
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Key words:
- surgical procedures, operative /
- sinusitis /
- hyperbaric oxygenation /
- nasal mucosa /
- inflammation
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[1] 韩德民.正确理解难治性鼻-鼻窦炎[J].中华耳鼻咽喉头颈外科杂志, 2013, 48(2):113-114.
[2] 赵澎, 雷静, 陈淡嫦, 等.胶原喷剂修复兔鼻黏膜的机械损伤[J].中国组织工程研究与临床康复, 2011, 15(51):9539-9543.
[3] EMING S A, KRIEG T, DAVIDSON J M.Inflammation in wound repair:molecular and cellular mechanisms[J].J Invest Dermatol, 2007, 127:514-525.
[4] 许庚, 李源, 谢民强, 等.功能性内窥镜鼻窦手术后术腔粘膜转归阶段的划分及处理原则[J].中华耳鼻咽喉科杂志, 1999, 34(5):302-305.
[5] WATELET J B, DEMETTER P, CLAEYS C, et al.Wound healing after paranasal sinus surgery:neutrophilic inflammation influences the outcome[J].Histopathology, 2006, 48:174-181.
[6] 中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组.慢性鼻-鼻窦炎诊断和治疗指南(2012年, 昆明)[J].中华耳鼻咽喉头颈外科杂志, 2013, 48(2):92-94.
[7] BOYCE J A.Mast cells:beyond IgE[J].J Allergy Clin Immunol, 2003, 111:24-32.
[8] 王晓燕, 张罗, 范尔忠, 等.正常钩突内外侧面黏膜炎性细胞的观察[J].临床耳鼻咽喉头颈外科杂志, 2011, 25(5):201-203.
[9] 高春锦, 杨捷云, 翟晓辉.高压氧医学基础与临床[M].北京:人民卫生出版社, 2008:397-399.
[10] THOM S R.Hyperbaric oxygen:its mechanisms and efficacy[J].Plast Reconstr Surg, 2011, 127 Suppl 1:131S-141S.
[11] YE X, ZHANG J, LU R, et al.HBO:apossible supplementary therapy for oral potentially malignant disorders[J].Med Hypotheses, 2014, 83:131-136.
[12] AL-WAILI N S, BUTLER G J.Effects of hyperbaric oxygen on inflammatory response to wound and trauma:possible mechanism ofaction[J].Sci World J, 2006, 3:425-441.
[13] 高春锦, 杨捷云, 翟晓辉.高压氧医学基础与临床[M].北京:人民卫生出版社, 2008:404-405.
[14] BROOK I.The role of bacteria in chronic rhinosinusitis[J].Otolaryngol Clin North Am, 2005, 38:1171-1192.
[15] 郭丽君.长期低剂量克拉霉素与甲硝唑治疗慢性鼻-鼻窦炎的观察与分析[J].临床耳鼻咽喉头颈外科杂志, 2011, 25(23):1098-1099.
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