CTLA-4在慢性扁桃体炎发病机制中的作用

周昌莉; 刘业海; 笪红婷; 昂倩倩; 沙泉

doi:10.13201/j.issn.1001-1781.2017.13.001

CTLA-4在慢性扁桃体炎发病机制中的作用

- 1. 安徽医科大学免疫学教研室及过敏与免疫研究中心(合肥, 230032);
- 2. 安徽医科大学第一附属医院耳鼻咽喉头颈外科;
- 3. 安徽医科大学第一附属医院检验科
基金项目:
国家自然科学基金（No：81273245）；2014年高等教育振兴计划人才项目基金

详细信息

通讯作者: 刘业海,E-mail:13856906986@126.com; 沙泉,E-mail:qsha2@yahoo.com

中图分类号: R766.18

收稿日期: 2017-04-20

The role of CTLA-4 in the pathogenesis of chronic tonsillitis

- 1. Department of Immunology, Allergy and Immunology Research Center, Anhui Medical University, Hefei, 230032, China;
- 2. Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Anhui Medical University;
- 3. Department of Clinical laboratory, the First Affiliated Hospital of Anhui Medical University

More Information

Corresponding authors: LIU Yehai ; SHA Quan

Received Date: 20 April 2017

摘要

摘要: 目的：探讨CTLA-4在慢性扁桃体炎发病中的作用，从炎症免疫反应软调节的角度为慢性扁桃体炎的药物治疗提供新的可能和理论依据。方法：从接受扁桃体全切术的患者中收取21例慢性扁桃体炎患者的扁桃体标本作为实验组，以15例扁桃体生理性肥大患者的扁桃体标本作为对照组。用苏木精-伊红染色法观察2组扁桃体组织的病理改变，用免疫荧光技术检测CTLA-4在2组中的表达差异和分布特征，用荧光定量PCR实验检测CTLA-4 mRNA在2组中的相对表达定量。结果：苏木精-伊红染色显示实验组扁桃体病理结构以滤泡增生和生发中心的变化为主要特征，对照组发现有明显的"星空"现象；免疫荧光结果显示，实验组扁桃体组织中CTLA-4的平均荧光强度和密度明显高于对照组（P<0.05）；荧光定量PCR实验结果显示，实验组CTLA-4 mRNA的相对表达量显著高于对照组（t=6.294，P<0.01）。结论： CTLA-4在慢性扁桃体炎的发展中起重要作用，说明慢性扁桃体炎症的转归过程中可能存在免疫抑制的机制。扁桃体淋巴组织存在免疫抑制，为临床上扁桃体反复发炎的现象提供了新的解释，同时为慢性扁桃体炎的临床治疗提供了除手术以外从免疫学角度研发新的治疗药物的可能性。
- CTLA-4 /
- 慢性扁桃体炎 /
- 免疫抑制 /
- 免疫治疗
Abstract: Objective: The purposes of the present study were to explore the role of CTLA-4 in the pathogenesis on development of chronic tonsillitis,and to provides new possibilities and theoretical basis for the drug therapy of chronic tonsillitis and, from soft regulation of the inflammatory immune response point of view.Method: Tonsil tissues were obtained by tonsillectomy and classified into two groups according to clinical forms of tonsillitis, CT(chronic tonsillitis) group(n=21) and TH (hypertrophy of tonsil)group(n=15). We used HE staining to observe the pathological changes in the structure of tonsil tissue in different groups. We used immunofluorescence(IF)method to determine the differences in expression of CTLA-4 between two groups and their distribution characteristics, and used Real-time fluorescent quantitative PCR technique(qRT-PCR) to detect CTLA-4 mRNA expression in the two groups.Result: ①HE staining showed the pathogeny structure mainly characterized as the follicular hyperplasia and germinal center in CT group,and obvious "star" phenomenons in TH group. ②Immunofluorescence results showed that the average fluorescence intensity and density of CTLA-4 protein in CT group was significantly higher than in TH group(P<0.05), the difference was statistically significant.③ The qRT-PCR experiment results showed that the expression level of CTLA-4 mRNA in CT group were obviously higher than in HT group (t=6.294,P<0.01), and the differences were statistically significant too.Conclusion: We found CTLA-4 played an important role in the development of chronic tonsillitis disease, suggesting that the mechanism of immunosuppression may exist in the process of chronic tonsil inflammation. Tonsil lymphoid tissue immune suppression provided a new explanation for recurrent tonsillitis, and provides a possibility for the development of new drugs for the treatment of chronic tonsillitis with the exception of surgery recurrent tonsillitis, at the same time for the clinical treatment of chronic tonsillitis in addition to surgery, but the possibility of researching new drugs from the perspective of immunology.
- CTLA-4 /
- chronic tonsillitis /
- immune suppression /
- immunotherapy

HTML全文

参考文献(14)

[1]	张小金.儿童扁桃体炎病因及发病机制研究进展[J].现代医药卫生, 2013, 29(4):551-553.
[2]	STELTER K.Indications and methods of tonsil surgery[J].MMW Fortscher Med, 2013, 155:39-42.
[3]	胡澜也, 杨军.扁桃体和(或)腺样体切除术后儿童免疫功能的变化[J].临床耳鼻喉头颈外科杂志, 2016, 30(5):418-423.
[4]	SUSAN M, TIFFANY H, AHARON G F, et al.Evidence for a stepwise program of extrathymic T cell development with in the human tonsil[J].J Clin Invest, 2013, 122:1403-1415.
[5]	张磊, 孙万邦.Th1/Th2型细胞因子与移植排斥及免疫耐受[J].医学综述, 2010, 4(16):501-503.
[6]	MARKOO J, VERICA A, PREDRAG V, et al.Ultrastructure of the human palatine tonsil and its functional significance[J].Rom J Morphol Embryol, 2015, 56:371-377.
[7]	AVRAMOVIC V, PETROVIC V, JOVIC M, et al.Quantification of cells expressing markers of proliferation and apoptosis in chronic tonsilitis[J].Acta Otorhinolaryngol Italica, 2015, 35:277-284.
[8]	NOBORU Y, SHIGEO S, YASUAKI H.Immunohistological study of tonsil:distribution of T cell subsets[J].Acta Otolaryngol, 2003, 96:509-516.
[9]	LUCY S K, DAVID M S.Confusing signals:recent progress in CTLA-4biology[J].Trends Immunol, 2015, 36:63-70.
[10]	TAN D B, FERNANDEZ S, PRICE P, et al.Impaired CTLA-4responses in COPD are associated with systemic inflammation[J].Cell Mol Immunol, 2014, 11:1-3.
[11]	CHRISTOPHER E R, ALISON T, HELGA S.CD28and CTLA-4coreceptor expression and signal transduction[J].Immunol Rev, 2009, 229:12-26.
[12]	WENDY A T, MARK G K, JOAQU M.A Molecular Perspective of CTLA-4Function[J].Ann Rev Immunol, 2006, 24:65-97.
[13]	魏伟.炎症免疫反应软调节[J].中国药理学通报, 2016, 32(3):297-303.
[14]	朱小慧, 盛德乔.免疫耐受机制的研究进展[J].细胞与分子免疫学杂志, 2010, 26(5):393-395.