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摘要: 目的:探讨喉鳞状细胞癌组织中Fra-1基因的表达情况。方法:应用免疫组织化学SP法及RT-PCR检测Fra-1在47例喉鳞状细胞癌组织和21例癌旁正常黏膜中的表达情况,进一步分析此基因在癌组织中表达与各临床病理参数的关系。结果:Fra-1基因在喉鳞状细胞癌组织中蛋白水平阳性表达率均高于癌旁正常黏膜组织(P＜0.05),Fra-1基因在喉鳞状细胞癌组织中蛋白水平表达与临床分期、淋巴结转移、吸烟相关,与病理分级、年龄、解剖分区均不相关;Fra-1基因在喉鳞状细胞癌组织中mRNA水平表达量均高于癌旁正常黏膜组织(P＜0.01),Fra-1基因在mRNA水平的表达与病理分级、临床分期、淋巴结转移、吸烟相关,与年龄、解剖分区无关。结论:喉鳞状细胞癌组织中Fra-1基因过度表达,此基因可能在喉癌发生、发展过程中发挥作用。Abstract: Objective: To investigate expressions of Fra-1 in laryngeal squamous cell carcinoma.Method: Using immunohistochemical method and RT-PCR detecting 47 cases of laryngeal squamous carcinoma tissues and 21 cases of para-carcinoma tissues with the level of protein and mRNA, we analysed the relationship of the genes expression and clinical parameters.Result: The level of protein positive expression of Fra-1 genes in laryngeal squamous cancer tissue were higher than adiacent tissues(P<0.05).The expression of Fra-1 protein in laryngeal squamous cancer tissue were significant correlation with the clinical stages, lymph metastasis, smoking. They had no significant correlation with the pathological grade, age, anatomic zonation.The mRNA level expression of Fra-1 genes in laryngeal squamous cancer tissue were higher than adjacent tissues(P<0.01). The expression of Fra-1 gene in the mRNA level was correlated with pathologic stage, clinical stage, lymph metastasis,and smoking, but wasn't correlated with age and anatomic zonation.Conclusion: In laryngeal squamous cell carcinoma, the expression of Fra-1 gene are overexpressed. Fra-1 may play a role in the occurrence and development of the laryngeal cancer.
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Key words:
- laryngeal neoplasms /
- carcinoma /
- squamous cell /
- immunohistochemistry /
- RT-PCR /
- Fra-1
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