OSAHS患者血清外周血干细胞生长因子和髓过氧化物酶的表达

廖礼兵; 翟锦明; 陈观贵; 马钊恩; 杨晓彬; 张建国

doi:10.13201/j.issn.1001-1781.2017.11.009

OSAHS患者血清外周血干细胞生长因子和髓过氧化物酶的表达

- 广州医科大学附属第二医院耳鼻咽喉科(广州, 510260)

详细信息

通讯作者: 翟锦明,E-mail:yyy1969120@163.com

中图分类号: R563.8

收稿日期: 2016-12-27

Expression and significance of serum hepatocyte growth factor and myeloperoxidase in patients with obstructive sleep apnea hypopnea syndrome

- Department of Otorhinolaryngology, the Second Affiliated Hospital of Guangzhou Medical University, 510260, China

More Information

Corresponding author: ZHAI Jinming, E-mail: yyy1969120@163.com

Received Date: 27 December 2016

摘要

摘要: 目的: 探讨OSAHS患者外周血干细胞生长因子(HGF)、髓过氧化物酶(MPO)的表达及意义。方法: 通过PSG确诊的96例OSAHS患者为OSAHS组,根据AHI范围分为重、中、轻3组;32例于我科体检健康者为对照组;ELISA法测定血HGF、MPO水平;予以重度OSAHS患者手术+CPAP方案治疗3个月并复测体内HGF、MPO浓度,分析HGF、MPO和PSG指标的相关性。结果: ①随着OSAHS病情程度增加,其外周血HGF和MPO浓度逐渐上升,不同程度各组患者HGF、MPO不全相同(F=119.006, 25.138;P＜0.01);其中轻度组与对照组间无明显差别(P>0.05);其余各组相互比较均有明显差别(P＜0.05)。②OSAHS患者血HGF、MPO浓度和年龄、BMI无关(P>0.05),而HGF与MPO浓度改变呈正相关,且二者均与AHI呈正相关,与LSaO₂呈负相关(P＜0.05)。③32例重度OSAHS患者经过3个月综合方案治疗后LSaO₂上升,AHI、外周血HGF、MPO水平降低,均有明显差别(P＜0.01)。治疗6个月后MPO、HGF浓度较治疗3个月时降低(P＜0.05),但和对照组相比仍有升高(P＜0.05)。结论: 手术联合较长时程CPAP治疗可降低OSAHS患者缺氧程度,降低心血管损害,其疗效和疗程相关;联合测定OSAHS患者外周血HGF与MPO对病情、疗效的判断及并发心血管损害的评估有一定的临床参考价值。
- 髓过氧化物酶 /
- 肝细胞生长因子 /
- 睡眠呼吸暂停低通气综合征,阻塞性
Abstract: Objective: The aim of this study is to explore the expression and significance of serum HGF and MPO in patients with obstructive sleep apnea hypopnea syndrome(OSAHS) in adults.Method: Ninety-six cases of OSAHS patients diagnosed by PSG were divided into 3 groups: Heavy, medium and light according to the range of AHI; 32 cases of outpatients were healthy persons as the control group. The serum HGF and MPO were determined by ELISA; HGF and MPO were measured after comprehensive treat with CPAP in patients with severe OSAHS, and the correlation between HGF, MPO and PSG were analyzed.Result: ①With the severity of OSAHS patients increased, the serum levels of HGF and MPO increased gradually(F=119.006, 25.138;P＜0.05);The concentration of HGF and MPO in each group showed that there was no significant difference between the two two groups compared with the control group(P>0.05); The rest of the two two groups were significantly different(P＜0.05).②There was no correlation between serum levels of HGF, MPO, BMI and age in OSAHS patients(P>0.05). The change of serum concentration was positively related to the two. All were negatively correlated with AHI, and positively correlated with LSaO₂ (P<0.05). ③LSaO₂ of 32 patients with severe OSAHS of has significantly increased after 3 months of comprehensive treatment, AHI and peripheral blood HGF, MPO levels were significantly reduced(P<0.05). The amylin and myeloperoxidase concentrations of blood after 6 months of treatment were significantly lower than those of 3 months after treatment(P<0.05), which still increased compared with the control group.Conclusion: Surgery combined with CPAP treatment can significantly reduce the degree of hypoxia and cardiovascular damage in OSAHS patients, which was significantly correlated with the treatment time. Combined detection of serum HGF and MPO concentrations in patients with OSAHS has a certain clinical value in judging the condition and curative effect and evaluating the cardiovascular damage.
- myeloperoxidase /
- hepatocyte growth factor /
- sleep apnea hypopnea syndrome,obstructive

HTML全文

参考文献(19)

[1]	SOMERS V K, WHITE D P, AMIN R, et al.Sleep apnea and cardiovascular disease:an American Heart Association/American College of Cardiology Foundation Scientific Statement from the American Heart Association Council for High Blood Pressure Research Professional Education Committee, Council on Clinical Cardiology, Stroke Council, and Council on Cardiovascular Nursing[J].J Am Coll Cardiol, 2008, 52:686-717.
[2]	王杰, 李莹, 林忠辉, 等.正压通气与上气道多平面手术治疗OSAHS研究进展[J].临床耳鼻咽喉头颈外科杂志, 2013, 27 (18):1036-1040.
[3]	GRIGORIEVA D V, GORUDKO I V, KOSTEVICH V A, et al.Plasma myeloperoxidase activity as a criterion of therapeutic effectiveness for patients with cardiovascular diseases[J].Biochem Suppl, 2016, 10:173-179.
[4]	邱承杰, 邱春光, 韩战营, 等.髓过氧化物酶在急性冠状动脉综合征危险分层中的作用[J].临床荟萃, 2010, 25 (7):603-604.
[5]	PIGUET A C, MEDOVM, KEOGH A, et al.Impact of MET targeting on tumor-associated angiogenesis and growth of MET mutations-drivenmodels of liver cancer[J].Genes Cancer, 2015, 6:317-327.
[6]	龚辉诚, 于锋, 颜永毅.成人阻塞性睡眠呼吸暂停低通气综合征外周血血栓调节蛋白和髓过氧化物酶的检测及其意义[J].临床耳鼻咽喉头颈外科杂志, 2016, 30 (20):1643-1646.
[7]	曹鄂洪, 朱美英, 施毅, 等.肝细胞生长因子在阻塞性睡眠呼吸暂停低通气综合征合并慢性阻塞性肺病的表达[J].医学研究生学报, 2013, 26 (9):941-943.
[8]	中华医学会呼吸病学分会睡眠呼吸障碍学组.阻塞性睡眠呼吸暂停低通气综合征诊治指南 (2011年修订版)[J].中华结核和呼吸杂志, 2012, 35 (1):9-12.
[9]	BROSTROM A, SUNNERGREN O, JOHANSSON P, et al.Symptom profile of undiagnosed obstructive sleep apnoea in hypertensive outpatients inprimary care:a structural equation model analysis[J].Qual Prim Care, 2012, 20:287-298.
[10]	KOMAROWSKA H, JASKULA M, STANGIERSKI A, et al.Influence of ghrelin on energy balance and endocrine physiology[J].Neuro Endocrinol Lett, 2012, 33:749-756.
[11]	AKINNUSI M E, LAPORTA R, EL-SOLH A A.Lectin-like oxidized low-density lipoprotein receptor-1modulates endothelial apoptosis inobstructive sleep apnea[J].Chest, 2011, 140:1503-1510.
[12]	刘成桂, 彭端亮, 罗俊, 等.联合检测MPO、IL-6和hsCRP对冠心病危险分层的价值[J].第三军医大学学报, 2011, 33 (19):2061-2065.
[13]	LIU J N, ZHANG J X, LU G, et al.The effect of oxidative stress in myocardial cell injury in mice exposed to chronic intermittent hypoxia[J].Chin Med J (Engl), 2010, 123:74-78.
[14]	ASSELBERGS F W, TERVAERT J W, TIO R A.Prognostic value of myeloperoxidase in patients with chest pain[J].N Engl J Med, 2004, 350:516-518.
[15]	BALDUS S, HEESCHEN C, MEINERTZ T, et al.Myeloperoxidase serum levels predict risk in patients with acute coronary syndromes[J].Circulation, 2003, 108:1440-1445.
[16]	PREZ-CALVO J I, MORALES-RULL J L, GIMENO-ORNA J A, et al.Usefulness of the Hepatocyte Growth Factor as a Predictor of Mortality in Patients Hospitalized With Acute Heart Failure Regardless of Ejection Fraction[J].Am J Cardiol, 2016, 118:543-549.
[17]	NISHIMURA Y, HYUGA S, TAKIGUCHI S, et al.Ephedrae herba stimulates hepatocyte growth factor-induced MET endocytosis anddownregulation via early/late endocytic pathways in gefitinib-resistant human lung cancer cells[J].Int J Oncol, 2016, 48:1895-1906.
[18]	HU Z P, BAO Y, CHEN D N, et al.Effects of recombinant adenovirus hepatocyte growth factor gene on myocardial remodelinginspontaneously hypertensive rats[J].J Cardiovasc Pharmacol Ther, 2013, 18:476-480.
[19]	LIANG M, PANG Y, ZHANG S, et al.Utility of Hepatocyte Growth Factor as a Biomarker for Early Diagnosis of Pulmonary ArteryHypertension[J].Mol Diagn Ther, 2016, 20:463-468.