青蒿琥酯对腺样囊性癌耐顺铂细胞裸鼠腮腺移植瘤的影响

徐新玲; 农晓琳; 陈虎; 陈宇麟

doi:10.13201/j.issn.1001-1781.2017.06.010

青蒿琥酯对腺样囊性癌耐顺铂细胞裸鼠腮腺移植瘤的影响

- 广西医科大学口腔医学院口腔颌面外科口腔颌面修复与重建研究实验室(广西壮族自治区重点实验室)
基金项目:
国家自然科学基金项目 (No:81360404)

广西自然科学基金项目 (No:2013GXNSFAA019231)

详细信息

通讯作者: 农晓琳, E-mail:Lab.nong@gmail.com

中图分类号: R789.97

收稿日期: 2016-11-24

Effect of artesunate on the transplantation tumor of parotid gland in the nude mice with adenoid cystic carcinoma drug resistance cell

- Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning, 530021, China

More Information

Corresponding author: NONG Xiaolin, E-mail: Lab.nong@gmail.com

Received Date: 24 November 2016

摘要

摘要: 目的: 观察青蒿琥酯对腺样囊性癌耐顺铂细胞(NACC/DDP)裸鼠腮腺移植瘤的抑瘤效果,探讨其作为抗癌药物的临床应用前景。方法: 选用5周龄雄性BALB/C鼠建立NACC/DDP裸鼠腮腺移植瘤模型,随机将裸鼠分成生理盐水对照组、DDP 2 mg/kg组、ART 200 mg/kg组、联合用药组(ART 100 mg/kg加DDP 1 mg/kg)共4组,隔日腹腔注射给药并测量各组裸鼠体重、移植瘤长短径。给药15次后取瘤,称瘤重,计算各用药组的抑瘤率,苏木精-伊红染色观察各组肿瘤组织病理改变并应用免疫组织化学方法检测Survivin在移植瘤组织中的表达情况。结果: 给药4周后DDP 2.0 mg/kg组的抑瘤率最高为59.86%(P<0.01),但该组裸鼠明显消瘦,状态不佳;ART 200 mg/kg 组的抑瘤率为43.54%(P<0.05),该组裸鼠精神状态良好,体重稳定增加;联合用药组的抑瘤率为41.16%(P<0.05),体重稍有减轻。苏木精-伊红染色结果显示:ART 200 mg/kg、DDP 2 mg/kg组和联合用药组肿瘤细胞核深染、固缩,核染色质浓缩、碎裂成片状,呈细胞凋亡状态,细胞间可见少量红染、无结构的坏死物。结论: 青蒿琥酯能够抑制腺样囊性癌耐药细胞(NACC/DDP)在裸鼠体内的增殖并促进其凋亡,且青蒿琥酯对裸鼠的毒副作用较顺铂小。
- 青蒿琥酯 /
- 腺样囊性癌 /
- 化疗耐药 /
- 移植瘤 /
- NACC/DDP细胞
Abstract: Objective: To observe the effect of Artesunate on the transplantation tumor of parotid gland in the nude mice with adenoid cystic carcinoma drug resistance cell and to explore its clinical application prospect as an anticancer drug.Method: Twenty male BALB/C mice were chose to set up parotid gland transplantation tumor model, then used the method of random Numbers table 20 nude mice were randomly divided into normal saline control group, positive control group DDP 2 mg/kg group, the ART of 200 mg/kg group, ART 100 mg/kg+DDP 1 mg/kg group, a total of 4 groups. Abdominal cavity injection was performed twice a day, and we measured weight of each nude mice and transplantation tumor major axis and minor axis. Treatments were conducted for 4 weeks, then the mice were sacrificed, and tumor weight was measured to calculate the inhibitory rates. The tumors were examined by HE staining to observe each tumor tissue pathological changes and by immunohistochemical method to detect Survivin expression in transplanted tumor tissue.Result: Four weeks after treatments, the inhibitory rate of DDP 2 mg/kg group was 59.86%, but this group of nude mice lost weight obviously; the ART 200 mg/kg group was 43.54%,this group of nude mice had a good mental state, and weight had gaining continuously;the ART 100 mg/kg+DDP 1 mg/kg group was 41.16%, this group of nude mice had reducing weight slightly.HE staining showed:The tumor nuclei were hyperchromatic and shrinkage, nuclear chromatin condensed and cracked into flake in the ART 200 mg/kg group, DDP 2 mg/kg group and the ART 100 mg/kg+DDP 1 mg/kg group.Conclusion: Artesunate can inhibit adenoid cystic carcinoma resistant cells NACC/DDP proliferation in nude mice in vivo and promote its apoptosis, and the side effects of Artesunate on nude mice were lower than Cisplatin.
- artesunate /
- adenoid cystic carcinoma /
- drug resistance /
- transplantation tumor /
- NACC/DDP cell

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