cAxin2调控鸡胚内耳早期发育

张小兵; Brand-Saberi B; 戴方平; 侯赟

doi:10.13201/j.issn.1001-1781.2016.11.011

cAxin2调控鸡胚内耳早期发育

- 1. 兰州大学第一医院耳鼻咽喉头颈外科(兰州, 730000);
- 2. 鲁尔大学解剖研究所解剖与分子胚胎研究室
基金项目:
甘肃省卫生厅行业科技计划项目（No：GSWST2011-060）；甘肃省中医药科学技术研究课题（No：GZK-2011-22）

详细信息

通讯作者: 张小兵,E-mail:790736924@qq.com

中图分类号: R764

收稿日期: 2015-10-30

The function of regulator cAxin2 in inner ear development of chick embry

- 1. Department of Otolaryngology Head and Neck Surgery, First Hospital of Lanzhou University, Lanzhou, 730000, China;
- 2. Department of Anatomy and Molecular Embryology, Institute of Anatomy, Ruhr University Bochum, 44780 Bochum, Germany

More Information

Corresponding author: ZHANG Xiaobing, E-mail: 790736924@qq.com

Received Date: 30 October 2015

摘要

摘要: 目的：探讨Wnt家族副调节因子cAxin2对鸡胚内耳早期发育的调控机制。方法：采用增强的绿荧光蛋白、cAxin2和短发夹RNA构建了一个带EGFP的干扰质粒，通过活体电穿孔技术转染在耳囊中，用原位杂交技术检测基因cAxin2、cPax2（Paired Box2）及cBMP4（Bone Morphogenetic Protein 4）在转染后耳囊中的表达。结果：转染侧耳囊显示cAxin2的表达被短发夹RNA抑制，cPax2被下调而cBMP4被上调，并能看到一个异常增大的耳囊。结论：cAxin2 是一个内耳发育过程中非常重要的调控基因。
- 鸡cAxin2 /
- 内耳发育 /
- RNA干扰
Abstract: Objective: To study the mechanism of the Wnt families'negative regulator cAxin2 in early inner ear development of chick embryo.Method: Plasmid was constructed with enhanced green fluorescent protein(EGFP), cAxin2 and short hairpin RNA(shRNA), which was transfected in otic vesicle by in ovo electroporation. Expression of cAxin2,cPax2(Pared box2),and cBMP4(bone morphogenetic protein 4) genes was investigated in the transfected oticvesicle with situ hybridization.Result: cAxin2 expression was inhibited by shRNA,cPax2 was down regulated and cBMP4 was up regulated as well as an abnormally enlarged otic vesicle was discovered in the transfected otic vesicle.Conclusion: cAxin2 is an important regulatory gene required for inner ear development.
- chick cAxin2 /
- inner ear development /
- RNA interference

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