FOXP3甲基化在变应性鼻炎中的作用研究

向荣; 刘昀; 许昱

doi:10.13201/j.issn.1001-1781.2016.09.009

FOXP3甲基化在变应性鼻炎中的作用研究

- 1. 武汉大学人民医院耳鼻咽喉头颈外科(武汉, 430060);
- 2. 武汉大学人民医院东院耳鼻咽喉头颈外科
基金项目:
国家自然科学基金（No：81371070）；湖北省自然科学基金（No：2014CKB495）；湖北省自然科学基金（No：2013CKB006）

详细信息

通讯作者: 许昱,E-mail:xy37138@163.com

中图分类号: R765.21

收稿日期: 2016-01-27

Effect of the FOXP3 gene methylation status in pathogenesis of patients with allergic rhinitis

- 1. Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China;
- 2. Department of Otolaryngology-Head and Neck Surgery, East Branch of Renmin Hospital of Wuhan University

More Information

Corresponding author: XU Yu, E-mail: xy37138@163.com

Received Date: 27 January 2016

摘要

摘要: 目的：研究变应性鼻炎（AR）患者和经变应原特异性免疫治疗（SIT）1年后患者外周血CD4⁺CD25⁺调节性T细胞FOXP3mRNA和FOXP3启动子基因甲基化水平，探讨FOXP3基因甲基化水平在AR发病机制及SIT作用机制中的作用。方法：以10例尘螨过敏的AR患者为AR组，10例屋尘螨过敏、经SIT 1年的AR患者为AR治疗组，10例正常体检者为对照组；分别取血备检。对患者进行视觉模拟量表（VAS）评分、荧光定量PCR及亚硫酸氢盐测序PCR（BSP法）检测外周血中CD4⁺CD25⁺调节性T细胞FOXP3mRNA及FOXP3基因启动子CpG岛甲基化状态。结果：AR治疗组VAS评分明显低于AR组；AR组FOXP3mRNA表达水平低于对照组和AR治疗组，差异有统计学意义（P<0.05）。对照组、AR组、AR治疗组CD4⁺CD25⁺调节性T细胞FOXP3基因启动子转录起始点上游的-127、-250位点CpG岛甲基化水平存在差异。AR组甲基化水平高于对照组和AR治疗组，差异有统计学意义（P<0.05）。结论：CD4⁺CD25⁺调节性T细胞FOXP3基因启动子序列-127、-250位点CpG岛甲基化水平升高可能与AR的发生、发展有关，SIT可能通过改变FOXP3基因甲基化水平调节患者的免疫功能从而起到治疗作用。
- 鼻炎,变应性 /
- CD4⁺CD25⁺调节性T细胞 /
- FOXP3 /
- 基因甲基化
Abstract: Objective: To analyze the mRNA expression level and the methylation status of FOXP3 gene in peripheral blood of patients with allergic rhinitis(AR) and explore the roles of FOXP3 gene in the pathogenesis of AR.Method: According to inclusion and exclusion criteria,10 AR patients,10 AR patients received SIT treatment for over one year,10 healthy controls were recruited for this study.Bisulfate sequencing technology(BSP) was used to detect the different methyation status of FOXP3 gene in peripheral blood between AR patients and controls. Real time fluorescence quantitative reverse transcription-polymerase chain reaction(RT-PCR) was used to detect different levels of FOXP3 mRNA in peripheral blood between AR patients and controls.Result: VAS scores of AR patients with SIT is much lower than that of AR patients. The expression levels of FOXP3 mRNA in AR patients are significantly lower compared to controls and AR patients with SIT(P<0.05). The methylation levels of AR is significantly higher compared to healthy controls and AR patients with SIT(P<0.05), whereas the methylation levels of AR patients with SIT is significantly higher compared to controls.Conclusion: The methylation levels of -127 and -250 CpG island on FOXP3 promoter in peripheral blood of AR patients may be associated with allergic rhinitis, and SIT may attenuate symptoms of AR by regulating the methylation levels of FOXP3 promoter.
- rhinitis, allergic /
- CD4⁺CD25⁺ regulatory T cells /
- Forkhead Box P3 /
- DNA methylation

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