神经生长因子对兔合并局部神经损伤的下颌骨骨折愈合中BMP-2表达的影响

吕桂浩; 蒋练; 满城; 向晓波

doi:10.13201/j.issn.1001-1781.2016.07.007

神经生长因子对兔合并局部神经损伤的下颌骨骨折愈合中BMP-2表达的影响

- 遵义医学院附属口腔医院(贵州遵义, 563003)
基金项目:
贵州省科技厅资助项目[No：黔科合J字LKZ（2010）03号]；贵州省遵义市红花岗区科技局资助项目[No：遵红科合社字LKZ（2009）10号]

详细信息

通讯作者: 蒋练,E-mail:jianglain1964@163.com

中图分类号: R338

收稿日期: 2015-12-07

The effect of nerve growth factor on the expression of BMP-2 in the healing of rabbits' mandibular fracture with partial nerve injury

- Stomatological Hospital Affiliated to Zunyi Medical College, Zunyi, Guizhou, 563003, China

More Information

Corresponding author: JIANG Lian, E-mail: jianglain1964@163.com

Received Date: 07 December 2015

摘要

摘要: 目的：通过鼠神经生长因子（NGF）在兔合并局部神经损伤的下颌骨骨折模型的局部应用，观察NGF对BMP-2表达的影响，初步探讨NGF促进合并局部神经损伤的下颌骨骨折愈合的分子机制。方法：将48只新西兰大白兔随机分为实验组（下颌骨骨折+切断神经束+以明胶海绵为载体的NGF）、对照组（下颌骨骨折+切断神经束+以明胶海绵为载体的NS）、空白组（下颌骨骨折+切断神经束）和完整组（下颌骨骨折+保留神经束）。手术建模后分别于2、4、6、8周每组各处死实验动物3只，苏木精-伊红染色观察下颌骨骨折愈合情况，荧光定量PCR检测BMP-2表达的改变。结果：苏木精-伊红染色观察到骨折区成骨现象：实验组较对照组明显，完整组较空白组明显，对照组与空白组相近。PCR早期结果差异有统计学意义，BMP-2表达在2周时达到最高峰后在中后期均呈下降趋势且下降幅度随时间减小。结论：局部应用NGF能够促使BMP-2在合并局部神经损伤的下颌骨骨折愈合的早期表达升高，这可能是NGF促进下颌骨骨折愈合的分子机制之一。
- 神经生长因子 /
- 下颌骨折 /
- BMP-2
Abstract: Objective: To observe the effect of NGF on the expression of BMP-2 in rabbit model and explore the molecular mechanism of NGF which might promote the healing of mandibular fracture with nerve injury.Method: The 48 New-Zealand white rabbits were randomly divided into the experimental group(mandibular fracture+to cut off the nerve bundle+NGF by GS), the control group(mandibular fracture+to cut off the nerve bundle+NS by GS), the blank group(mandibular fracture+to cut off the nerve bundle) and the full-set group(mandibular fracture+retains the nerve bundle). After 2 weeks, 4 weeks,6 weeks and 8 weeks,3 rabbits were sacrificed in each group for HE staining and RT-PCR, respectively.Result: HE staining showed the osteogenesis phenomenon:the experimental group was clearer than control group, the full-set group was clearer than the blank group and the control group is similarly to the blank group. RT-PCR results revealed that there was a statistically significance in the early stage. The expression of BMP-2 peaked in 2 weeks and decreased later with time.Conclusion: The local application of NGF can prompt BMP-2 expression in the early stages of the mandibular fracture with partial nerve injury healing and this may be one of the molecular mechanisms.
- nerve growth factor /
- mandibular fractures /
- BMP-2

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