MICA在喉癌组织和细胞中的表达及意义

王睿; 王郡甫; 苏庆红; 陈雪梅; 许晓群

doi:10.13201/j.issn.1001-1781.2016.02.002

MICA在喉癌组织和细胞中的表达及意义

- 1. 菏泽市立医院神经外科(山东菏泽, 274000);
- 2. 山东省医学科学院基础医学研究所;
- 3. 山东大学第二医院耳鼻咽喉科
基金项目:
国家自然科学基金青年基金(No:81202314)

山东省自然科学基金(No:ZR2013HL028,ZR2012HL23和ZR2010CM067)

山东省大型科学仪器升级改造技术研究专项(No:2013SJGZ09)

详细信息

通讯作者: 陈雪梅; 许晓群,E-mail:xuxiaoqunsd@163.com

中图分类号: R739.65

收稿日期: 2015-07-31

Expression and clinical significance of MICA in laryngeal carcinoma tissue and cells

- 1. Department of Neurosurgery, Heze Municipal Hospital, Heze, 274000, China;
- 2. Institute of Basic Medicine, Shandong Academy of Medical Sciences;
- 3. Department of Otolaryngology, the Second Hospital of Shandong University

More Information

Corresponding authors: CHEN Xuemei ; XU Xiaoqun

Received Date: 31 July 2015

摘要

摘要: 目的:探讨MICA在喉癌细胞Hep-2和喉鳞状细胞癌组织中的表达及意义。方法:RT-PCR和Western-blot方法检测MICA mRNA和蛋白在喉癌细胞和喉癌组织中的表达情况,并以喉外伤组织为对照。结果:RT-PCR检测结果显示MICA在Hep-2细胞中强势表达。与对照相比,MICA在喉癌组织中的表达均有增强(P<0.01),MICA表达强度与临床分期无关。Western-blot检测结果显示MICA蛋白在Hep-2细胞呈强势表达,但在喉癌组织中MICA蛋白随临床分期增高而表达降低。结论:MICA mRNA在Hep-2细胞和喉癌组织中均强势表达,其表达水平与临床分期无关。MICA蛋白在Hep-2细胞呈强势表达,但在喉癌组织中MICA蛋白随临床分期增高而表达降低。由此可见, MICA可能通过多种机制参与喉癌的发生、发展。
- MICA /
- Hep-2细胞 /
- 喉肿瘤
Abstract: Objective:To investigate the mRNA and protein expression of MICA in laryngeal squamous cell carcinoma tissue and the Hep-2 cells.Method:Reverse transcriptase-polymerase chain reaction(RT-PCR) and Western-blot were used to detect the expression of MICA mRNA and protein levels in the Hep-2 cells and laryngeal cancer tissues. Result:The MICA mRNA showed higher expression in Hep-2 cells by RT-PCR. Compared with the control, the mRNA expression of MICA was significantly enhanced in laryngeal cancer tissues (t=11.878, P<0.01). The intensity of MICA expression is not related to the clinical stage of cancer. MICA protein demonstrated higher level expression by Western blot. The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues.Conclusion:The MICA mRNA showed stronger expression in Hep-2 cells and laryngeal cancer tissues. The intensity of its expression is not related to clinical stage of cancer. The MICA protein expression was strong in Hep-2 cells. The intensity of MICA protein expression was decreased with increased clinical stage in laryngeal cancer tissues. MICA may play an important role in laryngeal carcinoma process.
- MICA /
- Hep-2cells /
- laryngeal neoplasms

HTML全文

参考文献(18)

[1]	KARATZANIS A D,PSYCHOGIOS G,WALDFAHRER F,et al.Management of locally advanced laryngeal cancer[J].J Otolaryngol Head Neck Surg,2014,43:4-4.
[2]	WONG T S,GAO W,LI Z H,et al.Epigenetic dysregulation in laryngeal squamous cell carcinoma[J].J Oncol,2012,2012:739461-739461.
[3]	林琳,余平.MICA/B分子研究进展[J].微生物学免疫学进展,2013,41(1):72-79.
[4]	CHEN Y,LIN G,GUO Z Q,et al.Effects of MICA expression on the prognosis of advanced non-small cell lung cancer and the efficacy of CIK therapy[J].PloS One,2013,8:e69044-e69044.
[5]	王筱静,王郡甫,刘琳,等.Toll样受体2在喉癌组织中的表达及意义[J].临床耳鼻咽喉头颈外科杂志,2013,27(12):629-632.
[6]	JEMAL A,BRAY F,CENTER M M,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61:69-90.
[7]	王睿,王郡甫,刘娣,等.白细胞介素-24对喉癌细胞的增殖效应及机制[J].临床耳鼻咽喉头颈外科杂志,2014,28(12):902-907.
[8]	ROMERO A I,CHAPUT N,POIRIER-COLAME V,et al.Regulation of CD4(+)NKG2D(+)Th1 cells in patients with metastatic melanoma treated with sorafenib:role of IL-15Rα and NKG2D triggering[J].Cancer Res,2014,74:68-80.
[9]	GASSER S,ORSULIC S,BROWN E J,et al.The DNA damage pathway regulates innate immune system ligands of the NKG2D receptor[J].Nature,2005,436:1186-1190.
[10]	JI M,WANG J,YUAN L,et al.MICA polymorphisms and cancer risk:a meta-analysis[J].Int J Clin Exp Med,2015,8:818-826.
[11]	WANG L P,NIU H,XIA Y F,et al.Prognostic significance of serum sMICA levels in non-small cell lung cancer[J].Eur Rev Med Pharmacol Sci,2015,19:2226-2230.
[12]	KOCH J,STEINLE A,WATZL C,et al.Activating natural cytotoxicity receptors of natural killer cells in cancer and infection[J].Trends Immunol,2013,34:182-191.
[13]	PARDOLL D M.Distinct mechanisms of tumor resistance to NK killing:of mice and men[J].Immunity,2015,42:605-606.
[14]	JIANG X,HUANG J F,HUO Z,et al.Elevation of soluble major histocompatibility complex class I related chain A protein in malignant and infectious diseases in Chinese patients[J].BMC Immunol,2012,13:62-62.
[15]	HENAGER S H,HALE M A,MAURICE N J,et al.Combining different design strategies for rational affinity maturation of the MICA-NKG2D interface[J].Protein Sci,2012,21:1396-1402.
[16]	HONG J,QIU T,QIAN T,et al.Heightened expression of MICA enhances the cytotoxicity of NK cells or CD8+T cells to human corneal epithelium in vitro[J].BMC Ophthalmol,2012,12:6-6.
[17]	RAULET D H,GASSER S,GOWEN B G,et al.Regulation of ligands for the NKG2D activating receptor[J].Annu Rev Immunol,2013,31:413-441.
[18]	CHITADZE G,BHAT J,LETTAU M,et al.Generation of soluble NKG2D ligands:proteolytic cleavage,exosome secretion and functional implications[J].Scand J Immunol,2013,78:120-129.