ZEB2和E-cadherin在鼻咽癌中的表达及其临床意义

易翔; 石书婧; 李晓雪; 赵利洁

doi:10.13201/j.issn.1001-1781.2015.18.016

ZEB2和E-cadherin在鼻咽癌中的表达及其临床意义

- 1 广西医科大学第一附属医院耳鼻咽喉头颈外科(南宁, 530021);
- 2 河北省眼科医院耳鼻咽喉科
基金项目:
广西医学科学实验中心开放基金(No:KFJJ2010-12)

详细信息

通讯作者: 易翔,E-mail:yixiang16300@163.Com

中图分类号: R739.63

收稿日期: 2015-06-11

Expression and clinical significance of ZEB2 and E-cadherin in nasopharyngeal carcinoma

- 1 Department of Otolaryngology-Head and Neck Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China;
- 2 Department of Otolaryngology, Hebei Province Ophthalmology Hospital

More Information

Corresponding author: YI Xiang, E-mail: yixiang16300@163.Com

Received Date: 11 June 2015

摘要

摘要: 目的:探讨ZEB2和E-cadherin mRNA和蛋白在鼻咽癌组织中的表达情况及其与鼻咽癌侵袭转移的关系。方法:应用荧光定量PCR和免疫组织化学方法检测 ZEB2和E-cadherin在39例鼻咽癌和12例炎性鼻咽黏膜组织中的表达,分析 ZEB2和 E-cadherin表达与鼻咽癌患者临床病理参数之间的关系及两者的相关性。结果:ZEB2 mRNA和蛋白在鼻咽癌中的表达水平均高于炎性鼻咽黏膜组织(P<0.05),ZEB2 mRNA和蛋白的高表达均与肿瘤的N分级和临床分期有关(P<0.05),而与性别、年龄和T分级无相关性(P>0.05);E-cadherin mRNA和蛋白在鼻咽癌中的表达水平均低于炎性鼻咽黏膜组织(P<0.05),E-cadherin mRNA和蛋白的低表达均与肿瘤的N分级有关(P<0.05),而与性别、年龄、T分级和临床分期无相关性(P>0.05);ZEB2 mRNA与E-cadherin mRNA呈负相关(r=-0.367,P<0.05),ZEB2 蛋白与E-cadherin 蛋白呈负相关(r=-0.322,P<0.05),均差异有统计学意义。结论:在鼻咽癌中ZEB2表达上调、E-cadherin表达下调,其表达呈明显负相关,并与鼻咽癌转移有关,可能机制与ZEB2下调E-cadherin的表达促进细胞EMT有关。
- 鼻咽癌 /
- 上皮-间质转化 /
- E盒结合锌指蛋白
Abstract: Objective: To investigate the expression and clinical significance of ZEB2 and E-cadherin mRNA and protein in nasopharyngeal carcinoma (NPC) tissues.Method: The expressions of ZEB2 and Ecadherin in 39 cases of NPC tissue and 12 cases of nasopharyngeal inflammation tissue were detected by Real-time PCR method and immunohistochemical technique. To assess their correlations with clinicopathological parameters of NPC and the interrelationship between them.Result: Both the expression of ZEB2 mRNA and protein were higher in NPC tissues than that in inflammation tissues (P<0.05). Abnormal expression of ZEB2 mRNA and protein in NPC were significantly associated to N stage and clinic stage (P<0.05), but the difference in expression between the different gender, age and T stage were not statistically significant (P>0.05). Both the expression of Ecadherin mRNA and protein were higher in NPC tissues than that in inflammation tissues (P<0.05). Abnormal expression of Ecadherin mRNA and protein in NPC were significantly associated to N stage (P<0.05), but the different expression between the different gender, age, T stage and clinic stage were not statistically significant (P>0.05). In NPC tissues, the expression of ZEB2 mRNA was negative correlated with the expression of E-cadherin mRNA (r=-0.367, P<0.05). The expression of ZEB1 protein was negative correlated with the expression of E-cadherin protein (r=-0.322, P<0.05), the differences were both statistically significant.Conclusion: The expression of ZEB2 was up-regulated in NPC, while the expression of E-cadherin was down-regulated, their expression was significantly negative correlated, and might be associated with metastasis of NPC, ZEB2 may promote the invasion and metastasis of NPC by inhibiting the expression of E-cadherin.
- nasopharyngeal carcinoma /
- epithelial-to-mesenchymal transition /
- zinc finger E-box-binding protein

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