高级检索

鼻咽癌组织中磷酸果糖激酶1蛋白表达及其酶活性检测

,  ,  ,  ,  , 

上一篇

下一篇

文章导航 > 临床耳鼻咽喉头颈外科杂志 > 2015 > 29(16): 1455-1457
李烁, 洪海裕, 杜政德, 等. 鼻咽癌组织中磷酸果糖激酶1蛋白表达及其酶活性检测[J]. 临床耳鼻咽喉头颈外科杂志, 2015, 29(16): 1455-1457. doi: 10.13201/j.issn.1001-1781.2015.16.011
引用本文: 李烁, 洪海裕, 杜政德, 等. 鼻咽癌组织中磷酸果糖激酶1蛋白表达及其酶活性检测[J]. 临床耳鼻咽喉头颈外科杂志, 2015, 29(16): 1455-1457. doi: 10.13201/j.issn.1001-1781.2015.16.011
shu
LI Shuo, HONG Haiyu, DU Zhengde, et al. Expression of phosphofructokinase 1 protein and it′s enzyme activity on nasopharyngeal carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surg, 2015, 29(16): 1455-1457. doi: 10.13201/j.issn.1001-1781.2015.16.011
Citation: LI Shuo, HONG Haiyu, DU Zhengde, et al. Expression of phosphofructokinase 1 protein and it′s enzyme activity on nasopharyngeal carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surg, 2015, 29(16): 1455-1457. doi: 10.13201/j.issn.1001-1781.2015.16.011
shu

鼻咽癌组织中磷酸果糖激酶1蛋白表达及其酶活性检测

    • 1 广东医学院附属深圳南山医院耳鼻咽喉科(深圳, 518052);

    • 2 中山大学附属第五医院耳鼻咽喉头颈外科

  • 基金项目:

    深圳市南山区科技局基金(No:2012031)

详细信息
    通讯作者: 高春生,E-mail:szgaocs@hotmail.com

  • 中图分类号: R739.63

Expression of phosphofructokinase 1 protein and it′s enzyme activity on nasopharyngeal carcinoma

    • 1 Department of Otolaryngology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, 518052, China;

    • 2 Department of Otolaryngology Head and Neck Surgery, the Fifth Hospital, Sun Yat-sen University

More Information

摘要

    摘要
  • 目的:研究糖酵解途径关键酶磷酸果糖激酶1(PFK1)的蛋白及其酶活性在鼻咽癌活检组织中的表达情况,并探讨其意义。方法:采取病例-对照设计的方法,实验组为41份鼻咽癌患者的鼻咽癌活检组织标本,对照组为20份鼻咽慢性炎性患者的鼻咽部活检组织标本,通过Western blot检测PFK1蛋白的表达;酶学分析的方法检测PFK1酶活性。结果:实验组的 PFK1蛋白表达及其酶活性水平均明显高于对照组(P<0.01);在实验组中,伴有淋巴结转移的鼻咽癌活检标本中的PFK1蛋白及其酶活性表达水平均明显高于无淋巴结转移的鼻咽癌活检标本(P<0.01)。结论:PFK1与鼻咽癌的发生、发展密切相关,可能作为鼻咽癌的肿瘤标志物之一。
    Objective: The aim of this study is to investigate the expression of phosphofructokinase 1 and it's enzyme activity in nasopharyngeal carcinoma biopsy samples.Method: Sixty-one biopsy samples were detected, including 41 tissues from patients with nasopharyngeal carcinoma as experimental group and 20 tissues from patients with chronic nasopharyngitis as control group. Phosphofructokinase 1 protein was detected by Western blot and it's enzyme activity was detected.Result: It was observed that the expression levels of phosphofructokinase 1 protein and it's enzyme activities in the experimental group were higher than that in the control group (P<0.01). In the experimental group, the expression levels of phosphofructokinase 1 protein and it's enzyme activities in patients with lymph node metastasis were higher than that in patients without lymph node metastasis(P<0.01).Conclusion: Phosphofructokinase 1 may be a marker in occurrence and metastasis of nasopharyngeal carcinoma.
    • HTML全文
  • 加载中
    • 参考文献(12)
  • [1]

    CHEN C,YI W,GAO J,et al.Alternative endpoints to the 5-year overall survival and locoregional control for nasopharyngeal carcinoma: A retrospective analysis of 2,450 patients[J]. Mol Clin Oncol,2014,2:385-392.

    [2]

    VANDER HEIDEN M G,CANTLEY L C,THOMPSON C B,et al.Understanding the Warburg effect: the metabolic requirements of cell proliferation[J].Science,2009,324:1029-1033.

    [3]

    LIU S,YAN B,LAI W,et al. As a novel p53 direct target, bidirectional gene HspB2/αB-crystallin regulates the ROS level and Warburg effect[J]. Biochim Biophys Acta,2014,1839:592-603.

    [4]

    ŠMERC A,SODJA E,LEGIŠA M.Posttranslational Modification of 6-phosphofructo-1-kinase as an Important Feature of Cancer Metabolism[J]. PloS One,2011,6:e19645-e19645.

    [5]

    WARBURG O.On the origin of cancer cells[J]. Science,1956,123:309-314.

    [6]

    SÁNCHEZ-ARAGÓ M,FORMENTINI L,CUEZVA J M. Mitochondria-mediated energy adaption in cancer: the H(+)-ATP synthase-geared switch of metabolism in human tumors[J]. Antioxid Redox Signal,2013,19:285-298.

    [7]

    MARÍN-HERNÁNDEZ A, GALLARDO-PÉREZ J C, RODRÍGUEZ-ENRÍQUEZ S,et al. Modeling cancer glycolysis[J]. Biochim Biophys Acta,2011,1807:755-767.

    [8]

    DAS L,VINAYAK M. Long Term Effect of Curcumin in Regulation of Glycolytic Pathway and Angiogenesis via Modulation of Stress Activated Genes in Prevention of Cancer[J].PLoS One,2015,10: e0124000-e0124000.

    [9]

    WEBER G. Enzymology of cancer cells (second of two parts)[J].N Engl J Med,1977,296:541-551.

    [10]

    YUAN Y,GUO-QING P, YAN T,et al. A study of PKM2, PFK-1, and ANT1 expressions in cervical biopsy tissues in China[J].Med Oncol,2012,29:2904-2910.

    [11]

    FUNATO Y,HAYASHI T,IRINO Y,et al. Nucleoredoxin regulates glucose metabolism via phosphofructokinase 1[J].Biochem Biophys Res Commun,2013,440:737-742.

    [12]

    李烁,杜政德,高进良,等.鼻咽癌组织中6-磷酸果糖激酶-1基因的表达及临床意义[J]. 临床耳鼻咽喉头颈外科杂志,2014,28(6):393-395.

    • 相关文章
  • 施引文献
  • 资源附件(0)
  • 加载中
    Created with Highcharts 5.0.7访问量Chart context menu近一年内文章摘要浏览量、PDF下载量统计信息摘要浏览量PDF下载量2024-072024-082024-092024-102024-112024-122025-012025-022025-032025-042025-052025-0600.511.522.5Highcharts.com
    Created with Highcharts 5.0.7Chart context menu访问类别分布HTML全文: 100.0 %HTML全文: 100.0 %HTML全文Highcharts.com
    Created with Highcharts 5.0.7Chart context menu访问地区分布其他: 12.2 %其他: 12.2 %上海: 1.2 %上海: 1.2 %十堰: 1.2 %十堰: 1.2 %华盛顿州: 2.4 %华盛顿州: 2.4 %南通: 1.2 %南通: 1.2 %嘉兴: 2.4 %嘉兴: 2.4 %天津: 6.1 %天津: 6.1 %张家口: 4.9 %张家口: 4.9 %扬州: 3.7 %扬州: 3.7 %杭州: 4.9 %杭州: 4.9 %桂林: 1.2 %桂林: 1.2 %武汉: 1.2 %武汉: 1.2 %温州: 1.2 %温州: 1.2 %漯河: 6.1 %漯河: 6.1 %石家庄: 3.7 %石家庄: 3.7 %芒廷维尤: 29.3 %芒廷维尤: 29.3 %芝加哥: 6.1 %芝加哥: 6.1 %西宁: 8.5 %西宁: 8.5 %邯郸: 2.4 %邯郸: 2.4 %其他上海十堰华盛顿州南通嘉兴天津张家口扬州杭州桂林武汉温州漯河石家庄芒廷维尤芝加哥西宁邯郸Highcharts.com
计量
  • 文章访问数:  238
  • PDF下载数:  156
  • 施引文献:  0
出版历程
收稿日期:  2015-05-14

目录

摘要
HTML全文
    参考文献(12)
    相关文章
    施引文献
    资源附件(0)
    返回

    用微信扫描二维码

    分享至好友和朋友圈

    友情链接

    二维码2

    版权所有 © 武汉协和医院杂志社 鄂ICP备 05004666-1 技术支持： 北京仁和汇智信息技术有限公司