BTG1在喉鳞状细胞癌组织中的表达及其临床意义

姜润学; 胡万宁; 孙国贵; 王建功; 韩晓晨

doi:10.13201/j.issn.1001-1781.2015.16.009

BTG1在喉鳞状细胞癌组织中的表达及其临床意义

- 唐山市人民医院生物治疗科(河北唐山, 063000)

详细信息

通讯作者: 胡万宁,E-mail:tangshanwann@163.com

中图分类号: R739.65

收稿日期: 2015-02-03

Expression of BTG1 protein in laryngeal squamous cell carcinoma and its clinical significance

- Department of Biological Treatment, Tangshan People's Hospital, Tangshan, 063000, China

More Information

Corresponding author: HU Wanning, E-mail: tangshanwann@163.com

Received Date: 03 February 2015

摘要

摘要: 目的:探讨B细胞易位基因1(BTG1)在喉鳞状细胞癌组织中的表达及其与喉鳞状细胞癌临床病理特征及生物学行为的关系。方法:采用免疫组织化学和Western blot检测70例喉鳞状细胞癌组织和35例癌旁组织中BTG1蛋白的表达水平。结果:BTG1蛋白在喉癌组织中的阳性表达率为31.43%,明显低于癌旁组织(91.43%, P<0.05)。Western blot示喉癌和癌旁组织BTG1相对表达量分别为0.217±0.032和0.918±0.081(P<0.05)。BTG1 在喉鳞状细胞癌组织中蛋白的表达与患者性别、年龄、肿瘤所在部位无关,与肿瘤局部浸润深度、区域淋巴结转移、临床分期、组织学分级呈负相关(P<0.05或P<0.01)。结论:在喉鳞状细胞癌组织中,BTG1蛋白的表达水平明显降低,检测BTG1的表达对喉癌的临床治疗和预后判断有一定的参考价值。
- B细胞易位基因1 /
- 喉肿瘤 /
- 免疫组织化学 /
- 蛋白质印迹
Abstract: Objective: To investigate the expression of B-cell translocation gene 1 (BTG1) and to determine the relationship between BTG1 expression and clinicopathological features, biological behaviors in laryngeal squamous cell carcinoma.Method: Immunohistochemistry and Western blot were used to analyze BTG1 protein expression in 70 cases of laryngeal cancer and 35 cases of adjacent corresponding laryngeal mucosal tissues to illuminate the relationship between BTG1 expression and clinical factors.Result: The positive rate of BTG1 protein expression was 31.43% in laryngeal carcinoma tissues, significantly lower than 91.43% in the adjacent laryngeal tissues (P<0.05). Western blot showed the relative expression of BTG1 protein between cancer lesion and adjacent tissue were 0.217± 0.032 and 0.918±0.081, showing the difference with statistical significance (P<0.05). The expression of protein was significantly correlated with the tumor invasion, lymph node metastasis, clinic stage and histological grade (P<0.05 or P<0.01), but not with sex, age and tumor location (P>0.05) of patients with laryngeal cancer.Conclusion: The expression of BTG1 protein was decreased in laryngeal squamous cell carcinoma, suggesting that BTG1 gene may be closely associated with the carcinogenesis and the degree of malignancy. Detection of BTG1 expression may be useful in diagnosis, treatment and prognosis of laryngeal carcinoma.
- B-cell translocation gene 1 /
- laryngeal neoplasms /
- immunohistochemistry /
- Western blot

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