血清总IgE、INF-γ、IL-4作为变应性鼻炎动物模型建立的客观评价指标初探

张建辉; 唐嗣泉

doi:10.13201/j.issn.1001-1781.2015.15.013

血清总IgE、INF-γ、IL-4作为变应性鼻炎动物模型建立的客观评价指标初探

张建辉,
唐嗣泉^,

- 川北医学院附属医院耳鼻咽喉头颈外科(四川南充, 637000)
基金项目:
四川省教育厅资助课题(No:12ZB221

11ZA185)

四川省卫生厅资助课题(No:110295)

川北医学院2012年度科研发展计划项目[No:川北医科(2012)17号

详细信息

通讯作者: 唐嗣泉,E-mail:tangsiqu@126.com

中图分类号: R765.21

收稿日期: 2015-04-14

Establishment and evaluation of the SD rat allergic rhinitis model

ZHANG Jianhui,
TANG Siquan^,

- Department of Otorhinolaryngology-Head and Neck Surgery, the Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, China

More Information

Corresponding author: TANG Siquan, E-mail: tangsiqu@126.com

Received Date: 14 April 2015

摘要

摘要: 目的:探讨血清总IgE、INF-γ、IL-4作为卵清白蛋白致敏实验性变应性鼻炎(AR)大鼠模型建立客观评价指标及意义。方法:以SD大鼠为实验对象,随机分为AR组和对照组,每组10只。AR组以卵清白蛋白作为致敏原,对照组以生理盐水替代卵清白蛋白,其余方法与步骤2组均相同。采用行为学评分、鼻腔组织学观察及血清IgE、IL-4、IFN-γ检测综合判断造模是否成功。结果:2组鼻腔激发后行为学评分差异有统计学意义(P<0.05)。对大鼠鼻黏膜进行苏木精-伊红染色,AR组大鼠鼻黏膜符合AR形态学改变,对照组大鼠鼻黏膜变化并不明显。血清总IgE、IL-4、IFN-γ ELISA检测结果,2组血清中IL-4、IgE含量比较,AR组显著高于正常对照组,差异有统计学意义(P<0.01)。AR组血清中INF-γ含量显著低于正常对照组,差异有统计学意义(P<0.01)。结论:卵清白蛋白致敏成功建立AR大鼠模型,血清总IgE、INF-γ、IL-4含量可作为建立AR动物模型的客观评价指标。
- 鼻炎 /
- 变应性 /
- 大鼠模型 /
- IgE /
- INF-γ /
- IL-4
Abstract: Objective: To investigate method established and system evaluated in the model of SD rat with AR.Method: To establish AR model of SD rats by ovalbumin (OVA), 20 cases of SD rats were randomly divided into two groups, namely control group (10 cases) and AR group (10 cases). AR models were sensitized and challenged by OVA.Control group were used with normal saline instead of OVA. The score of pathology and praxiology were observed when the SD rats in AR group appeared typical symptom of allergic rhinitis ,and levels of IL-4, IFN-γ, IgE in the serum were examined by ELISA. According to the behavioral score, nasal histology and content of IL-4, IFN-γ, IgE of serum, Rat allergic rhinitis model were judged successfully established or not. Result: Behavioral scores were significantly increased in OVA-challenged rats compared with the control group,P<0.05. Nasal epithelial goblet cells, eosinophils and lymphocytes in nasal mucosa in the AR rats exhibited obvious increase relative to the control group. IL-4, IgE levels in the AR rat exhibited obvious increase relative to control group while INF-γ levels exhibited obvious reduction (P<0.05). Conclusion: The allergic rhinitis models in SD rat by OVA were successfully established. The levels of IgE,INF-γ and IL-4 in Serum can be used as objective evaluation of animal models of allergic rhinitis established successfully or not.
- allergic rhinitis /
- rat model /
- IgE /
- INF-γ /
- IL-4 /

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