VIP及其受体在不同程度慢性酒精中毒大鼠耳蜗的表达差异及其意义

冯静; 刘海兵

doi:10.13201/j.issn.1001-1781.2015.14.017

VIP及其受体在不同程度慢性酒精中毒大鼠耳蜗的表达差异及其意义

冯静,
刘海兵^,

- 成都大学附属医院耳鼻咽喉头颈外科(成都, 610000)

详细信息

通讯作者: 刘海兵,E-mail:liuhb99@126.com

中图分类号: R322.9

收稿日期: 2015-04-09

The expression and significance of VIP and its receptor in the cochlea of different degrees of chronic alcoholism rats

FENG Jing,
LIU Haibing^,

- Department of Otorhinolaryngology-Head and Neck Surgery, the Affiliated Hospital of Chengdu University, Chengdu, 610000, China

More Information

Corresponding author: LIU Haibing, E-mail: liuhb99@126.com

Received Date: 09 April 2015

摘要

摘要: 目的:探讨不同程度的慢性酒精中毒对大鼠耳蜗神经肽VIP(血管活性肠肽)及其受体表达的影响及其机制。方法:30只SD大鼠随机分为3组进行造模:轻度酒精中毒组、重度中毒组及对照组。采用RT-PCR分别检测3组大鼠耳蜗VIP的表达,采用Western blotting检测大鼠耳蜗VIP受体蛋白的表达,采用免疫组织化学的方法定位及定量检测大鼠耳蜗VIP的表达。结果:慢性酒精中毒组的大鼠耳蜗VIP及其受体的表达下调,并且重度慢性酒精中毒的大鼠耳蜗VIP及其受体的表达低于轻度组,差异有统计学意义(P<0.05)。慢性酒精中毒组的大鼠耳蜗VIP及其受体与对照组在神经节细胞的表达差异无统计学意义(P>0.05)。结论:VIP及其受体在慢性酒精中毒大鼠的听觉系统功能中发挥着重要的作用。慢性酒精中毒可引起听觉系统的一种肽类激素分泌不平衡,最终导致听力损失。
- chronic alcoholism /
- cochlea /
- rat /
- vasoactive intestinal polypeptide
Abstract: Objective: To determine whether chronic alcoholism alters the expression levels of Vasoactive intestinal polypeptide (VIP) and its receptor(VIPR1) in the cochlea of chronic alcoholism rats.Method: We measured their expression levels in 30 SD rats, in which we created models of different degrees of chronic alcoholism. We investigated the presence of the mRNA of VIP in the cochlea of chronic alcoholism rats and controls by reverse transcription-polymerase chain reaction (RT-PCR) method. We investigated the presence of proteins of VIPR1 in poisoned rats and controls by western blot. We also evaluated the local distribution of VIP cells by immunohistochemistry.Result: We found that the levels of VIP and VIPR1 were downregulated in the chronic alcoholism groups compared to the controls group. The differences in some expression levels were significant different between chronic alcoholism rats and control rats. Moreover, at different degrees of alcohol poisoning in rats, the contents of VIP and VIPR1 differed. Decreased levels of VIP and VIPR1 were detected in the deep chronic alcoholism group compared to the group with low-degree poisoning (P<0.05). In spiral ganglion cell plasm the expression of VIP and VIPR1 had no significant difference in three groups (P>0.05).Conclusion: These results suggest that VIP and VIPR1 play an important role in the auditory function in rats with chronic alcoholism. Chronic alcoholism may cause a peptide hormone secretion imbalance in the auditory system, eventually leading to hearing loss.
- chronic alcoholism /
- cochlea /
- rat /
- vasoactive intestinal polypeptide

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