大鼠变应性鼻炎鼻黏膜AMCase mRNA的表达

刘燕; 邢志敏; 王旻

doi:10.13201/j.issn.1001-1781.2015.03.021

大鼠变应性鼻炎鼻黏膜AMCase mRNA的表达

- 北京大学人民医院耳鼻咽喉科(北京, 100044)
基金项目:
北京市科技计划课题(No:Z131107002213056)

详细信息

通讯作者: 邢志敏,E-mail:xing_zhimin@tom.com

中图分类号: R765.21

收稿日期: 2014-08-25

Expression of AMCase mRNA in nasal mucosa of allergic rhinitis in SD rats

- Department of Otolaryngology, Peking University People's Hospital, Beijing, 100044, China

More Information

Corresponding author: XING Zhimin, E-mail: xing_zhimin@tom.com

Received Date: 25 August 2014

摘要

摘要: 目的:研究变应性鼻炎(AR)大鼠鼻黏膜AMCase mRNA表达,探讨其与AR的关系。方法:30只SD大鼠,随机分为实验组和对照组。实验组20只,卵清白蛋白(OVA)隔日腹腔注射基础致敏2周,继之每日鼻腔局部激发1周,建立大鼠AR模型;对照组10只,生理盐水代替OVA进行实验。末次激发后根据鼻部症状累积评分评判AR造模是否成功,并取所有大鼠双侧鼻腔及中隔黏膜,常规苏木精-伊红染色病理明确AR诊断。提取鼻黏膜RNA,应用反转录合成cDNA,采用实时荧光定量 PCR(RT-PCR)检测AMCase mRNA在大鼠AR鼻黏膜中的表达。结果:RT-PCR 检测AMCase mRNA表达,显示实验组与对照组差异有统计学意义(P＜0.05);②AR组AMCase mRNA表达同鼻部症状评分正相关(r=0.411,P＜0.05)。结论:大鼠AR鼻黏膜AMCase mRNA表达量增多,提示AMCase表达上调在变应性鼻炎的发病机制中起作用,抑制该酶活性可以成为AR治疗新的靶点。
- 鼻炎 /
- 变应性 /
- 酸性哺乳动物几丁质酶 /
- 分子生物学
Abstract: Objective: To examine AMCase mRNA expression levels in nasal mucosa of allergic rhinitis SD rats.Method: Thirty SD rats were chosen and randomly divided into the experimental group and the control group. 20 in experimental group and 10 in the control group.AR model rats were established through repeated intraperitoneal shot of ovalbumin (OVA) for 2 weeks and consequently confirmed by local challenge with OVA for 1 week. The control group was treated by the same method with Physiological saline water instead of OVA. After the last excitation allergic rhinitis was diagnosed according to the accumulation score about nasal symptom. The septal mucosa of all rats were used to diagnose pathologically by HE dyeing. AMCase mRNA in nasal mucosa, obtained from the bilateral nasal mucosa in two groups, were used to do reverse transcriptive polymerase chain reaction.Real-time polymerase chain reaction was used to examine AMCase mRNA expression levels.Result: ①The results showed definitely that there were positive expression of AMCase mRNA in normal nasal mucosa. This expression increase significantly during nasal allergy(P＜0.05).②The increased expression of AMCase mRNA in allergic rhinitis are related with the nasal symptoms score (r=0.411,P＜0.05).Conclusion: Increased expression of AMCase mRNA in nasal mucosa in allergic rhinitis model might play roles in the pathogenesis of AR,and Restrain the enzyme activity could become new treatment targets of allergic rhinitis.
- rhinitis /
- allergic /
- acid mammals chitinase /
- molecular biology

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