YKL-40在变应性鼻炎患者血清中的表达及与IL-4和IFN-γ相关性分析

刘飞; 李烁; 高春生

doi:10.13201/j.issn.1001-1781.2015.03.019

YKL-40在变应性鼻炎患者血清中的表达及与IL-4和IFN-γ相关性分析

- 鼻咽喉科(广东深圳, 518000)

详细信息

通讯作者: 高春生:E-mail:szgaocs@hotmail.com

中图分类号: R765.21

收稿日期: 2014-06-03

Expression of serum YKL-40 in anaphylactic rhinitis patients and association of serum YKL-40 with serum IL-4 and IFN-γ

- Department of Otorhinolaryngology, Shenzhen Nanshan Hospital, Shenzhen, 518000, China

More Information

Corresponding author: GAO Chunsheng, E-mail: szgaocs@hotmail.com

Received Date: 03 June 2014

摘要

摘要: 目的:比较不同类型变应性鼻炎(AR)患者血清中YKL-40的表达水平,分析其与IL-4、IFN-γ的相关性,探讨其在AR发病机制中的可能作用。方法:选取轻度间歇性、轻度持续性、中-重度间歇性、中-重度持续性AR患者各20例作为实验组,正常健康者20例作为对照组。应用酶联免疫吸附法检测各组血清中YKL-40、IL-4、IFN-γ的表达水平。对以上各组数据进行统计学分析。结果:①不同实验组与对照组血清YKL-40相比表达均明显升高,差异均有统计学意义(P<0.05)。轻度持续组与轻度间歇组血清YKL-40相比表达升高,差异有统计学意义(P<0.05)。中-重度持续组与中-重度间歇组血清YKL-40相比表达升高,差异有统计学意义(P<0.05)。在轻度间歇组与中-重度间歇组、轻度持续组与中-重度持续组之间表达差异均无统计学意义(P>0.05)。②不同实验组与对照组血清IL-4相比表达均明显升高,差异均有统计学意义(P<0.05)。各实验组之间血清IL-4表达差异均无统计学意义(P>0.05)。③不同实验组与对照组血清IFN-γ相比表达均明显降低,差异均有统计学意义(P<0.05)。各实验组之间血清IFN-γ表达差异均无统计学意义(P>0.05)。④AR血清YKL-40水平升高与血清IL-4水平呈正相关,与血清IFN-γ呈负相关。结论:①AR血清中YKL-40表达升高,且与IL-4水平呈正相关,与IFN-γ水平呈负相关,提示YKL-40可能通过调节Th细胞的分化,促进Th1向Th2的免疫偏移,参与AR的发病。②与间歇性AR患者相比,持续性AR患者血清中YKL-40表达升高,提示YKL-40可能会促进AR症状的持续发作。
- YKL-40 /
- IL-4 /
- IFN-γ /
- 鼻炎 /
- 变应性
Abstract: Objective: This study intends to preliminarily discuss the expression and the clinical meaning of serum YKL-40 in anaphylactic rhinitis by comparing the different expressions of serum YKL-40 in types of anaphylactic rhinitis, and to preliminarily discuss possible mechanism of having anaphylactic rhinitis involved with serum YKL-40 by associating it with serum IL-4 and IFN-γ in anaphylactic rhinitis.Method: Firstly, each select 20 people in our hospital who have anaphylactic rhinitis respectively according to different levels--mild intermittent, mild persistent, moderate-severe interminttent, moderate-severe persistent as the experimental groups, while 20 healthy people as the nomal control. Secondly, test the levels of serum YKL-40, IL-4 and IFN-γ respectively in each group by the ELISA method. Thirdly, statistically analyze and discuss the collected data.Result: ①There was a rise in the expression of serum YKL-40 between the experimental groups and the nomal control group, which contained a statistical significance (P<0.05). There was a rise in the expression of serum YKL-40 between the mild persistent group and mild intermittent group, which contained a statistical significance (P<0.05), as well as between the moderate-severe persistent group and the moderate-severe intermittent group (P<0.05), while there was no significant difference in expression of serum YKL-40 between the mild intermittent group and the moderate-severe interminttent group, as well as between the mild persistent group and the moderate-severe persistent group (P>0.05).②There was a rise in the expression of serum IL-4 between the experimental groups and the nomal control group, which contained a statistical significance (P<0.05), while there was no significant difference between each experimental group (P>0.05).③There was a reduction in the expression of serum IL-4 between the experimental groups and the nomal control group, which contained a statistical significance (P<0.05), while there was no significant difference between each experimental group (P>0.05).④The corelation between serum YKL-40 and IL-4 in anaphylactic rhinitis was positive, while the corelation between serum YKL-40 and IFN-γ was negative. Conclusion: ①Allergic rhinitis serum YKL-40 expression was increased.The corelation between serum YKL-40 and IL-4 in anaphylactic rhinitis was positive, while the corelation between serum YKL-40 and IFN-γ was negative,suggesting that YKL-40 may regulate the differentiation of Th cells, Promote Th1 to Th2 immune shift, through this mechanism involved in the pathogenesis of allergic rhinitis.②Compared with patients with intermittent allergic rhinitis, persistent allergic rhinitis serum YKL-40 expression was increased, suggesting that YKL-40 may promote the continuous attacks of the symptoms of allergic rhinitis.
- YKL-40 /
- IL-4 /
- IFN-γ /
- rhinitis /
- allergic

HTML全文

参考文献(10)

[1]	JOHANSEN J S, JENSEN B V, ROSLIND A, et al. Is YKL-40 a new therapeutic target in cancer[J]?Expert Opin Ther Targets,2007,11:219-234.
[2]	KUCUR M, ISMAN F K, KARADAG B, et al. Serum YKL-40 levels in patients with coronary artery disease[J]. Coron Artery Dis,2007,18:391-396.
[3]	JOHANSEN J S. Studies on serum YKL-40 as a biomarker in diseases with inflammation,tissue remodelling,fibroses and cancer[J]. Dan Med Bull,2006,53:172-209.
[4]	REHLI M, KRAUSE S W, ANDREESEN R. Molecular characterization of the gene for human cartilage gp-39(CHI3L1), a member of the chitinase protein family and marker for late stages of macrophage differentiation[J]. Genomics,1997,43:221-225.
[5]	LEE C G, ELIAS J A. Role of breast regression protein-39/YKL-40 in asthma and allergic responses[J]. Allergy Asthma Immunol Res,2010,2:20-27.
[6]	SPECJALSKI K, JASSEM E. YKL-40 protein is a marker of asthma[J]. J Asthma,2011,48:767-772.
[7]	PARK J A, DRAZEN J M, TSCHUMPERLIN D J. The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress[J]. J Biol Chem,2010,285:29817-29825.
[8]	TANG H, FANG Z, SUN Y, et al. YKL-40 in asthmatic patients, and its correlations with exacerbation,eosinophils and immunoglobulin E[J]. Eur Respir J,2010,35:757-760.
[9]	许庚,张罗,高志强,等. 2009年世界鼻科学大会侧记[J].中华耳鼻咽喉头颈外科杂志,2009,44(7):612-613.
[10]	KWON J W, KIM T W, CHO S H, et al. Serum YKL-40 levels are correlated with symptom severity in patients with allergic rhinitis[J]. Allergy,2011,66:1252-1253.