RT-PCR检测鼻咽癌患者外周血细胞以及癌组织细胞中CK18和CK19基因的表达

黄铄; 李双; 彭涛; 吴婷婷; 宋鹏; 周绪红

doi:10.13201/j.issn.1001-1781.2015.02.004

RT-PCR检测鼻咽癌患者外周血细胞以及癌组织细胞中CK18和CK19基因的表达

- 1 武汉大学中南医院耳鼻咽喉科(武汉, 430000);
- 2 武昌中南医院耳鼻咽喉科

详细信息

通讯作者: 周绪红,E-mail:zhouxuhong62@126.com

中图分类号: R739.63

收稿日期: 2014-08-05

Detection of cytokeratin18 and cytokeratin19 gene expression in blood and tumor tissue of nasopharyngeal carcinoma patients by RT-PCR

- 1 Department of Otorhinolaryngology, Zhongnan Hospital of Wuhan University, Wuhan, 430000, China;
- 2 Department of Otorhinolaryngology, Zhongnan Hospital of Wuchang

More Information

Corresponding author: ZHOU Xuhong, E-mail: zhouxuhong62@126.com

Received Date: 05 August 2014

摘要

摘要: 目的:通过检测CK18和CK19在鼻咽癌患者外周血细胞和癌组织细胞中的表达情况,判断其是否可以作为鼻咽癌的肿瘤标志物辅助诊断鼻咽癌。方法:分别提取45例鼻咽癌患者的外周血样本中的有核细胞和癌组织细胞中的mRNA,逆转为cDNA,设计CK18、CK19和GAPDH引物进行RT-PCR,检测其表达情况。结果:在不同性别、年龄以及转移与否的鼻咽癌患者癌组织细胞中CK18和CK19 基因的表达的均差异无统计学意义(P>0.05),而在不同病理分期的鼻咽癌患者癌组织细胞中,CK18和CK19基因表达随着恶性程度的增高而增高,且均差异有统计学意义(P<0.05);在对鼻咽癌患者的外周血中的CK18和CK19 基因检出率结果显示,CK18的检出率为64%,CK19的检出率75%,年龄大于60岁的患者检出率高于年龄小于60岁的,随着病理检测其恶性程度的增高,CK18和CK19的检出率也相应增高,鼻咽癌转移的患者血液中CK18和CK19的检出率远高于非转移患者。结论:CK18和CK19是可能有潜力作为鼻咽癌诊断的肿瘤标志物。
- CK18 /
- CK19 /
- RT-PCR /
- 鼻咽癌
Abstract: Objective: To measure the expression of CK18 and CK19 in the cells from peripheral blood and tumor tissue of the nasopharyngeal carcinoma patients,to test whether CK 18 and CK 19 could be biomarkers of nasopharyngeal carcinoma fordiagnosis.Method: The mRNA was extracted from the blood and carcinoma tissue of nasopharyngeal carcinoma and was reversed transcription to cDNA. The 3 pairs primers were designed for RT-PCR and the fold value was calculated to evaluated expression by △CT. Result: There are no statistical differences between the CK18 and CK19 gene expression and the gender, age and metastasis in tumor tissue of 45 nasopharyngeal carcinoma patients (P>0.05). There are significant differences among 3 pathological stages and 2 genes expressed increase as the grade malignancy (P<0.05).The detecting of the 2 genes expression from blood cells shows that CK18 and CK19 had a high positive ratio 64% and 75% respectively. Meanwhile this method showed a same detection characteristic in tumor and blood, the positive rate of CK18 and CK19 genes in metastasis is higher than non-metastasis. The results showed CK18 has a high specificity and CK19 has a high sensitivity for prognosis and all relapsed cases are associated with the expression of CK18 and CK19.Conclusion: CK18 and CK19 may be used as biomarkers of nasopharyngeal carcinoma for diagnosis.
- cytokeratin 18 /
- cytokeratin 19 /
- real time-polymerase chain reaction /
- nasopharyngeal carcinoma

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