衔接蛋白AP-2蛋白在小鼠耳蜗中的表达及意义

顾翔; 宋锐; 陈知己; 袁伟

doi:10.13201/j.issn.1001-1781.2015.01.020

衔接蛋白AP-2蛋白在小鼠耳蜗中的表达及意义

- 第三军医大学附属西南医院耳鼻咽喉头颈外科(重庆, 400038)
基金项目:
国家自然科学基金资助项目(No:8147069,No:30973301,No:81271080)

重庆市自然基金资助项目(No:CSTC2009BB5170)

详细信息

通讯作者: 袁伟,E-mail:weiyuan175@sina.com

中图分类号: R764.35

收稿日期: 2014-10-22

The expression and significance of adaptin-2 in mice cochlea

- Department of Otorhinolaryngology-Head and Neck Surgery, Southwest Hospital of Third Military Medical University, Chongqing, 400038, China

More Information

Corresponding author: YUAN Wei, E-mail: weiyuan175@sina.com

Received Date: 22 October 2014

摘要

摘要: 目的:研究衔接蛋白AP-2在小鼠耳蜗中的定位与表达,探讨AP-2蛋白与内毛细胞胞吞的相关性。方法:选择健康成年C57BL/6J小鼠,应用免疫荧光标记技术结合激光共聚焦显微镜观察AP-2蛋白在其耳蜗毛细胞的定位与表达。结果:AP-2蛋白主要定位于小鼠耳蜗内毛细胞细胞质,集中表达于内毛细胞基底部与带状突触靠近传入神经元区域。结论:AP-2蛋白在成年小鼠耳蜗中主要表达于内毛细胞突触活化部位,可能与内毛细胞突触囊泡的内吞作用相关,为深入研究AP-2蛋白在内耳听觉生理和病理机制中的作用奠定了基础。
- 网格蛋白 /
- 衔接蛋白 /
- 内吞 /
- 内毛细胞
Abstract: Objective: To investigate the expression of adaptin-2(AP-2) in mice cochlea and to discuss the probable role in the endocytosis of hair cells.Method: Laser scanning confocal microscopy and immune-fluroscence histochemistry were performed in this study.Result: In mature mice cochlea, the immunoreactivity for AP-2 was found in the inner hair cells cytoplasm. This protein mainly expressed in the hair cells basal part and nearby the ribbon synapse.Conclusion: AP-2 protein mainly expressed in the hair cells synaptic activity zone, which suggested that AP-2 could play an important role in the synaptic vesicle endocytosis. This finding built the foundation for the further research involved in the physiological and pathological role of AP-2.
- clathrin /
- adaptin /
- endocytosis /
- inner hair cell

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参考文献(13)

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