联合应用以Hif-1α和Survivin基因为靶标的siRNA对鼻咽癌CNE-2细胞增殖和周期的影响

任艳鑫; 张书萍; 李晓江; 杨洁; 孙瑞梅; 赵留芳; 李磊

doi:10.13201/j.issn.1001-1781.2014.24.019

联合应用以Hif-1α和Survivin基因为靶标的siRNA对鼻咽癌CNE-2细胞增殖和周期的影响

- 1 昆明医科大学第三附属医院头颈肿瘤研究中心(昆明, 650118);
- 2 四川省人民医院城东病区耳鼻咽喉科
基金项目:
2012年国家自然科学基金地区项目(No:81260312)

2011年云南省卫生科技内设研究机构项目资助(No:2011WS0068)

云南省社会发展科技计划(应用基础研究重点项目)(No:2009CC026)

详细信息

通讯作者: 李晓江,E-mail:wingman11@163.com

中图分类号: R739.63

收稿日期: 2014-01-06

Combined application the siRNA target for Hif-1α and Survivin gene to human NPC CNE-2 cell and its effects on the proliferation and cycle of this cell

- 1 Head and Neck Tumor Research Center of Third Affiliated Hospital of Kunming Medical University, Kunming, 650118, China;
- 2 Department of Otorhinolaryngology in the East City Ward, Sichuan Province People's Hospital, Chengdu

More Information

Corresponding author: LI Xiaojiang, E-mail: wingman11@163.com

Received Date: 06 January 2014

摘要

摘要: 目的:研究联合转染以乏氧诱导因子-1α(Hif-1α)和Survivin基因为靶标的siRNA后,鼻咽癌CNE-2细胞增殖和周期的改变。方法:以Hif-1α和Survivin基因为靶标的siRNA共同转染鼻咽癌CNE-2细胞,以单靶点siRNA转染组和未处理组为对照。MTT检测转染后细胞的增殖活力;流式细胞仪检测转染后细胞的周期变化,RT-PCR与Western Blot技术检测转染后各组细胞相应靶基因mRNA及相关蛋白表达水平的变化。结果:鼻咽癌CNE-2细胞分别转染以Hif-1α和Survivin基因为靶标的siRNA后,细胞的增殖能力明显下降,相应靶基因的mRNA及蛋白的表达明显减低,细胞周期出现G₀/G₁期阻滞;联合转染两种siRNA后细胞的各项检测结果均明显强于对照组,与各组相比差异均有统计学意义(P<0.01)。结论:联合以Hif-1α和Survivin基因为靶标的siRNA干扰组,可以较单基因干扰组更有效干扰鼻咽癌CNE-2细胞内靶基因mRNA和蛋白的表达,抑制细胞的增殖。
- 鼻咽肿瘤 /
- RNA干扰 /
- 乏氧诱导因子-1α /
- 存活素
Abstract: Objective:To study the combined transfection of the siRNA target for Hif-1α and Survivin gene to human NPC CNE-2 cell and its effects on the proliferation and cycle of this cell.Method:Combined transfection of the siRNA target for Hif-1α and Survivin gene to human NPC CNE-2 cell, these plasmids were respectively transfected into the same cells. Cell proliferation was detected with MTT assay. The inhibitory effects on target genes were evaluated with RT-PCR and Western Blot at the levels of mRNA and protein, respectively. Result:MTT showed that CNE-2 cell proliferation in multi-gene plasmid group was more significantly inhibited than a single gene. The expression of mRNA and protein of two different genes were both decreased in HS group, and the interference effect of multiple genes was better than the single-gene(P<0.01). Conclusion:HS group could restrain cell proliferation and interference the mRNA and protein expression in nasopharyngeal carcinoma CNE-2 cell, which was better than the other groups.
- nasopharyngeal neoplasms /
- RNAi /
- Hif-1α /
- Survivin

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