L-多巴脱羧酶在喉癌中的表达

赖史胜; 万志荔

doi:10.13201/j.issn.1001-1781.2014.24.016

L-多巴脱羧酶在喉癌中的表达

赖史胜¹,
万志荔^2, ,

- 1 赣南医学院第一附属医院耳鼻咽喉科(江西赣州, 341000);
- 2 赣南医学院第一附属医院眼科
基金项目:
赣州市指导性科技计划项目(No:GZ2014ZSF086)

详细信息

通讯作者: 万志荔,E-mail:kw856@sina.com

中图分类号: R739.65

收稿日期: 2014-07-21

Research of expression of L-DOPA decarboxylase in laryngeal cancer

LAI Shisheng¹,
WAN Zhili^2, ,

- 1 Department of Otorhinolaryngology, the First Affiliated Hospital of Gannan Medical College, Ganzhou, 341000, China;
- 2 Department of Ophthalmology, the First Affiliated Hospital of Gannan Medical College

More Information

Corresponding author: WAN Zhili, E-mail: kw856@sina.com

Received Date: 21 July 2014

摘要

摘要: 目的:分析L-多巴脱羧酶(DDC)mRNA和蛋白在喉癌组织中的表达,以评估其在喉癌诊断和预后中的临床意义。方法:从53例喉癌患者的肿瘤切除手术中获取106份组织样品,并从这些组织样品中分别分离出总RNA。采用RT-PCR法对DDC mRNA的表达水平进行量化,同时采用Western Blot蛋白质印迹法对DDC蛋白的表达水平进行检测。结果:与正常喉黏膜组织相比,原发性喉癌(P=0.000)和复发性喉癌(P=0.033)样品中的DDC mRNA表达水平都出现了显著下调。且DDC mRNA表达水平与患者年龄和组织学分级无关,但与TNM分期之间存在显著的负相关性(P=0.021)。同时,我们在肿瘤和正常喉组织中都检测到了DDC蛋白的表达。结论:DDC的表达状态可能在喉癌的发展中发挥重要的作用,在未来的喉癌患者临床管理中具有重要的意义。
- 喉肿瘤 /
- L-多巴脱羧酶 /
- 相对定量RT-PCR /
- 印迹法 /
- 蛋白质
Abstract: Objective:This study aimed to investigate the expression levels of L-DOPA decarboxylase (DDC) mRNA and protein in laryngeal cancer, and to determine the clinical significance of DDC in diagnosis and prognosis of laryngeal cancer.Method:Total RNA was isolated from 106 tissue samples surgically removed from 53 laryngeal cancer patients. A quantitative real-time polymerase chain reaction (RT-PCR) methodology based on SYBR Green I fluorescent dye was developed for the quantification of mRNA levels. In addition, Western Blot analysis was performed to detect the expression level of DDC protein.Result:DDC mRNA expression in both primary (P=0.000) and recurrent (P=0.033) laryngeal cancer samples downregulated significantly compared with their nonmalignant counterparts. Moreover, expression of DDC mRNA was not associated with age and histologic grade, but the significantly decreased mRNA were correlated with early TMN stage (P=0.021). Additionally, DDC protein was detected in both cancerous and noncancerous tissues.Conclusion:Expression levels of DDC may play a vital role in the progression of laryngeal cancer, which can be served as a promising biomarker for the future clinical management of laryngeal cancer patients.
- laryngeal neoplasms /
- L-DOPA decarboxylase /
- quantitative RT-PCR /
- Blotting /
- Western

HTML全文

参考文献(14)

[1]	LICITRA L, BERNIER J, GRANDI C, et al.Cancer of the larynx[J].Crit Rev Oncol Hematol, 2003, 47:65-80.
[2]	MIRISOLA V, MORA R, ESPOSITO A I, et al.A prognostic multigene classifier for squamous cell carcinomas of the larynx[J].Cancer Lett, 2011, 307:37-46.
[3]	BOZZI F, LUKSCH R, COLLINI P, et al.Molecular detection of dopamine decarboxylase expression by means of reverse transcriptase and polymerase chain reaction in bone marrow and peripheral blood:utility as a tumor marker for neuroblastoma[J].Diagn Mol Pathol, 2004, 13:135-143.
[4]	KONTOS C K, PAPADOPOULOS I N, FRAGOULIS E G, et al.Quantitative expression analysis and prognostic significance of L-DOPA decarboxylase in colorectal adenocarcinoma[J].Br J Cancer, 2010, 102:1384-1390.
[5]	LIVAK K J, SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) Method[J].Methods, 2001, 25:402-408.
[6]	GEOMELA P A, KONTOS C K, YIOTAKIS I, et al.L-DOPA decarboxylase mRNA expression is associated with tumor stage and size in head and neck squamous cell carcinoma:a retrospective cohort study[J].BMC Cancer, 2012, 12:484-484.
[7]	RUBIB, MAECHLER P.Minireview:new roles for peripheral dopamine on metabolic control and tumor growth:let's seek thebalance[J].Endocrinology, 2010, 151:5570-5581.
[8]	CHAKROBORTY D, SARKAR C, BASU B, et al.Catecholamines regulate tumor angiogenesis[J].Cancer Res, 2009, 69:3727-3730.
[9]	GANGULY S, BASU B, SHOME S, et al.Dopamine, by acting through its D2receptor, inhibits insulin-like growth factor-Ⅰ (IGF-Ⅰ) -induced gastric cancer cell proliferation via up-regulation of Krüppellike factor 4through down-regulation of IGF-IR and AKT phosphorylation[J].Am J Pathol, 2010, 177:2701-2707.
[10]	HE Q, YUAN L B.Dopamine inhibits proliferation, induces differentiation and apoptosis of K562leukaemia cells[J].Chin Med J (Engl), 2007, 120:970-974.
[11]	AVGERIS M, KOUTALELLIS G, FRAGOULIS E G, et al.Expression analysis and clinical utility of LDopa decarboxylase (DDC) in prostate cancer[J].Clin Biochem, 2008, 41 (14-15):1140-1149.
[12]	SAKAKURA C, TAKEMURA M, HAGIWARA A, et al.Overexpression of dopa decarboxylase in peritoneal dissemination of gastric cancer and its potential as a novel marker for the detection of peritoneal micrometastases with real-time RT-PCR[J].Br J Cancer, 2004, 90:665-671.
[13]	GOULIOUMIS A K, VARAKIS J, GOUMAS P, et al.Androgen receptor in laryngeal carcinoma:could there be an androgen-refractory tumor[J]?ISRN Oncol, 2011, 2011:180-518.
[14]	MARGIOTTI K, WAFA L A, CHENG H, et al.Androgen-regulated genes differentially modulated by the androgen receptor coactivator L-dopa decarboxylase in human prostate cancer cells[J].Mol Cancer, 2007, 6:38-38.