siRNA沉默C-erbB-2基因对人喉癌Hep-2细胞PI3K/Akt信号通路的影响

余文发; 赵玉林; 王萍; 马慧敏; 张国正; 鲁保才; 董明敏

doi:10.13201/j.issn.1001-1781.2014.12.017

siRNA沉默C-erbB-2基因对人喉癌Hep-2细胞PI3K/Akt信号通路的影响

- 1. 新乡医学院第一附属医院耳鼻咽喉头颈外科(河南新乡, 453100);
- 2. 郑州大学第一附属医院耳鼻咽喉头颈外科;
- 3. 新乡医学院第三附属医院功能检查科
基金项目:
河南省高等学校青年骨干教师资助计划（No：2010GGJS-120）和河南省卫生科技创新人才工程（No：2010141）联合资助

详细信息

通讯作者: 余文发,E-mail:yuwenfa197288@aliyun.com

中图分类号: R739.65

收稿日期: 2013-11-22

The effects of C-erbB-2 siRNA on PI3K/Akt signaling pathway in human laryngeal cancer Hep-2 cells

- 1. Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Xinxiang Medical College, Xinxiang, 453100, China;
- 2. Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Zhengzhou University;
- 3. Department of Functional Examination, the Third Affiliated Hospital of Xinxiang Medical College

More Information

Corresponding author: YU Wenfa, E-mail: yuwenfa197288@aliyun.com

Received Date: 22 November 2013

摘要

摘要: 目的：探讨siRNA沉默人喉癌Hep-2细胞C-erbB-2基因对细胞增殖的影响，及其与PI3K/Akt信号通路的关系。方法：利用siRNA 技术转染Hep-2细胞C-erbB-2基因，RT-PCR3检测siRNA转染后C-erbB-2 mRNA的水平变化，MTT检测细胞增殖情况改变，Western blot检测ERK1/2和AKT1蛋白磷酸化变化情况。结果：转染C-erbB-2 siRNA 24 h后的Hep-2细胞C-erbB-2 mRNA水平出现下调，细胞增殖抑制率增加，ERK1/2和AKT1蛋白磷酸化水平也相应降低，C-erbB-2基因表达与ERK1/2和AKT1蛋白磷酸化水平之间有显著相关性。结论：siRNA沉默Hep-2细胞C-erbB-2抑制Hep-2细胞增殖，这种增殖抑制可能通过降低ERK1/2和AKT1蛋白磷酸化水平实现。
- 喉肿瘤 /
- C-erbB-2基因 /
- ERK1/2 /
- AKT1
Abstract: Objective: To investigate the effects of short interfering RNA(siRNA) silencing C-erbB-2 gene on the proliferation of laryngeal carcinoma Hep-2 cells and to explore the relationship between C-erbB-2 gene and PI3K/Akt signal pathway. Method: C-erbB-2 siRNA was transfected to Hep-2 cells, and mRNA expression of C-erbB-2 was detected by RT-PCR. MTT was applied to detect the proliferation of Hep-2 cells. Western blot was used to detect the phosphorylation changes of protein ERK1/2 and AKT1. Result: The mRNA level of C-erbB-2 in Hep-2 cells declined obviously at 24 h after transfection of C-erbB-2 siRNA. The proliferation of transfected Hep-2 cells was inhibited, and protein phosphorylation levels of ERK1/2 and AKT1 decreased simultaneously. The expression of C-erbB-2 gene was obviously correlated with the protein phosphorylation levels of ERK1/2 and AKT1. Conclusion: C-erbB-2 silence by siRNA can efficiently inhibit the proliferation of Hep-2 cells, which may be achieved by decreasing the phosphorylation level of ERK1/2 and AKT1.
- laryngeal neoplasms /
- C-erbB-2 gene /
- ERK1/2 /
- AKT1

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