连翘酯苷对顺铂作用后豚鼠耳蜗c-jun表达的影响

安宁; 时胜武

doi:10.13201/j.issn.1001-1781.2014.10.018

连翘酯苷对顺铂作用后豚鼠耳蜗c-jun表达的影响

安宁^,,
时胜武

- 沈阳医学院附属奉天医院耳鼻咽喉科(沈阳, 110024)

详细信息

通讯作者: 安宁,E-mail:lilian77@126.com

中图分类号: R762

收稿日期: 2013-07-06

The effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig

AN Ning^,,
SHI Shengwu

- Department of Otolaryngology, Fengtian Hospital Affiliated to Shenyang Medical College, Shenyang, 110024, China

More Information

Corresponding author: AN Ning, E-mail: lilian77@126.com

Received Date: 06 July 2013

摘要

摘要: 目的：研究连翘酯苷对顺铂作用后豚鼠耳蜗c-jun表达的影响。方法：将30只豚鼠随机分为对照组（10只），顺铂组（10只）和连翘酯苷组（10只）。腹腔注射顺铂溶液（8 mg/kg），1次/d，连续7 d，建立顺铂耳毒性模型；连翘酯苷组在每次注射顺铂溶液30 min前腹腔注射连翘酯苷25.0 mg/kg/d，连续7 d；对照组以生理盐水代替顺铂溶液注射，连续7 d。实验动物被处死前，检测其DPOAE幅值变化；采用蛋白质印迹杂交（Western Blotting）检测各组豚鼠耳蜗c-jun蛋白的表达，逆转录聚合酶链反应检测各组豚鼠耳蜗c-jun基因mRNA的表达。结果：顺铂组DPOAE幅值明显低于对照组（P<0.01）；相比于顺铂组，连翘酯苷组DPOAE幅值明显升高（P<0.05）。顺铂组豚鼠耳蜗c-jun蛋白与mRNA表达水平均显著高于对照组（P<0.01）；相比于顺铂组，连翘酯苷组c-jun蛋白与mRNA表达水平明显降低（P<0.05）。结论：连翘酯苷能够通过降低c-jun的表达防护顺铂所致的耳蜗损伤。
- 顺铂 /
- 连翘酯苷 /
- 耳毒性 /
- c-jun /
- 豚鼠
Abstract: Objective: To study the effect of forsythiaside on the expression of c-jun induced by cisplatin in the cochlea of guinea pig. Method: Thirty guinea pigs were randomly divided into control group (10), cisplatin group (10) and forsythiaside group (10). The ototoxicity model was done with intraperitoneal injection of cisplatin solution (8 mg/kg per day) for 7 days. Forsythiaside (25 mg/kg per day) was injected 30 min before cisplatin solution treated in guinea pigs of forsythiaside group for 7 consecutive days.The saline instead of cisplatin was injected in normal control group. The distortion product otoacoustic emission (DPOAE) was detected before animals were killed.The expression of c-jun in cochlea of guinea pigs was detected by western blotting. The expression of c-jun mRNA in cochlea of guinea pigs was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Result: DPOAE amplitudes in cisplatin group was significantly lower than in control group (P<0.01). Compared with cisplatin group, DPOAE amplitudes in forsythiaside group was increased significantly (P<0.05). The expression of c-jun protein and mRNA were significantly increased in cisplatin group than in control group (P<0.01). Compared with cisplatin group, the expression of c-jun protein and mRNA were significantly decreased in forsythiaside group. Conclusion: Forsythiaside can significantly reduce the side effects induced by cisplatin through down-regulating the expression of c-jun.
- cisplatin /
- forsythiaside /
- ototoxicity /
- c-jun /
- guinea pig

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