FA-MNP-MMP-9-ASODN纳米复合物对鼻咽癌的体外抑制研究

刘涛; 谢民强; 许熠铭; 张宏征; 张涛

doi:10.13201/j.issn.1001-1781.2014.09.007

FA-MNP-MMP-9-ASODN纳米复合物对鼻咽癌的体外抑制研究

- 广州南方医科大学珠江医院耳鼻咽喉头颈外科(广州, 510282)
基金项目:
国家自然科学基金资助项目 (No:81372477, No:81260406)

教育部高等学校博士学科点专项科研基金 (No:20114433110001)

详细信息

通讯作者: 谢民强, E-mail:min_qiang_x@hotmail.com

中图分类号: R739.6

收稿日期: 2013-11-03

The inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma in vitro

- Department of Otolaryngology-Head and Neck Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China

More Information

Corresponding author: XIE Minqiang, E-mail: min_qiang_x@hotmail.com

Received Date: 03 November 2013

摘要

摘要: 目的:观察FA-MNP-MMP-9-ASODN纳米复合物对鼻咽癌HNE-1细胞的体外抑制效应。方法:采用RT-PCR、Western-blot方法、MTT方法、流式细胞技术及Matrigel体外侵袭实验观察转染FA-MNP-MMP-9-ASODN 48 h后HNE-1细胞MMP-9 mRNA及蛋白表达水平和细胞增殖能力、凋亡和侵袭能力的变化。结果:FA-MNP-MMP-9-ASODN组HNE-1细胞MMP-9 mRNA和蛋白的表达水平较对照组及FA-MNP-MMP-9-NSODN无义序列组明显降低。同时FA-MNP-MMP-9-ASODN组HNE-1细胞生长抑制率约为35.66%,增殖活性较对照组和无义序列组明显降低。且HNE-1细胞周期也受到抑制,细胞凋亡率约为12.60%,明显高于对照组和无义序列组。侵袭实验显示FA-MNP-MMP-9-ASODN组穿膜细胞数约为21.00个,明显低于对照组和无义序列组。结论:FA-MNP-MMP-9-ASODN纳米复合物通过抑制MMP-9的表达,可降低鼻咽癌细胞的增殖和侵袭能力,促进凋亡,体外抑制效果良好。
- 鼻咽肿瘤 /
- 基质金属蛋白酶9 /
- 反义寡核苷酸 /
- 纳米复合物
Abstract: Objective:To observe the inhibitory effect of FA-MNP-MMP-9-ASODN nanocomposite on nasopharyngeal carcinoma(NPC)HNE-1 cell in vitro. Method:To observe the MMP-9 mRNA and protein expression levels, proliferation ability,cell apoptosis and invasion ability of HNE-1 cell 48 hours after FA-MNP-MMP-9-ASODN transfection by RT-PCR, Western-blot,MTT assay,flow cytometry and Matrigel invasion test. Result:MMP-9 mRNA and protein expression in HNE-1 cell of FA-MNP-MMP-9-ASODN group was significantly decreased compared to control group and nonsense sequence group of FA-MNP-MMP-9-NSODN. At the same time, for the HNE-1 cell in FA-MNP-MMP-9-ASODN group, growth inhibition rate was about 35.66%, proliferation activity significantly decreased compared to the control group and the nonsense sequence group, cell cycle was also inhibited, cell apoptosis rate was about 12.60%, the apoptosis rate was significantly higher than that in the control group and the nonsense sequence group. Invasion assay showed that the transmembrane cells in FA-MNP-MMP-9-ASODN group were about 21.00,significantly lower than that in the control group and the nonsense sequence group. Conclusion:By inhibiting the expression of MMP-9, FA-MNP-MMP-9-ASODN nanocomposites could reduce NPC cell proliferation and invasion ability, and promote apoptosis, it had a good inhibitory effect in vitro.[JP]
- nasopharyngeal carcinoma /
- matrix metalloproteinase 9 /
- antisense oligonucleotide /
- nanocomposite

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