咽喉反流对声门型喉癌中COX-2mRNA表达的影响

吕艳春; 王丽萍; 王思宁

doi:10.13201/j.issn.1001-1781.2014.06.001

咽喉反流对声门型喉癌中COX-2mRNA表达的影响

- 中国医科大学附属盛京医院耳鼻咽喉科(沈阳, 110004)
基金项目:
辽宁省自然基金 (No:20092135)

辽宁省教育厅高校科技研究项目 (No:2009A757)

详细信息

通讯作者: 王丽萍, E-mail:wanglp@sj-hospital.org

中图分类号: R739.65

收稿日期: 2013-06-05

Laryngopharyngeal reflux effect on expression of COX-2mRNA in glottic carcinoma

- Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Shenyang, 110004, China

More Information

Corresponding author: WANG Liping, E-mail: wanglp@sj-hospital.org

Received Date: 05 June 2013

摘要

摘要: 目的:探讨咽喉反流对环加氧酶2(COX-2)mRNA 在声门型喉癌病变黏膜中表达的影响。方法:对40例声门型喉癌患者行电子鼻咽喉镜检查、反流检查计分量表(RFS)和反流症状指数量表(RSI)评分,将其分为声门型喉癌反流阳性组(20例)和声门型喉癌反流阴性组(20 例),另取10例癌旁2 cm以上黏膜组织作为对照组。采用Real-time PCR方法,测定各组黏膜中COX-2mRNA的表达情况。结果:声门型喉癌组COX-2mRNA的表达明显高于对照组(P<0.05); 声门型喉癌反流阳性组的表达高于声门型喉癌反流阴性组(P<0.05)。结论:咽喉反流因素可能通过组织损伤、炎症及细胞恶性转化使声门型喉癌的COX-2mRNA的表达升高。
- 环加氧酶 /
- 咽喉反流 /
- 声门型喉癌
Abstract: Objective:To investigate laryngopharyngeal reflux effect on expression of COX-2mRNA in glottis carcinoma lesion mucosa.Method:Forty cases with glottic laryngeal cancer were examined by electronic naspharyngeal laryngoscope and scored by the reflux symptom index (RSI) and the reflux finding score(RFS). Based on the scores, they were divided into two groups:glottic laryngeal cancer with positive reflux(20 cases) and glottic laryngeal cancer with negative reflux(20 cases). Ten cases with adjacent normal membrane were used as control group. The mRNA expression of COX-2 from 40 patients was examined by reverse transcription polymerase chain reaction(RT-PCR).Result:The expression of COX-2mRNA in tumor samples was significantly higher than that in normal tissues(P<0.05); the expression of COX-2mRNA in glottic laryngeal cancer with positive reflux was significantly higher than that in glottic laryngeal cancer with negative reflux(P<0.05).Conclusion:Laryngopharyngeal reflux factors may increased expression glottic carcinoma of COX-2mRNA by tissue injury, inflammation and cell malignant transformation.
- cyclooxygenase /
- laryngopharyngeal reflux /
- glottic carcinoma

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