乙酰肝素酶、蛋白激酶CK2β与缺氧诱导因子-1α在鼻咽癌组织中的表达及其相关性研究

钟启龙; 王双乐; 李创伟; 杨楚; 林炘; 林歆胜; 卢汉桂

doi:10.13201/j.issn.1001-1781.2014.03.005

乙酰肝素酶、蛋白激酶CK2β与缺氧诱导因子-1α在鼻咽癌组织中的表达及其相关性研究

- 汕头市中心医院耳鼻咽喉头颈外科(广东汕头, 515031)
基金项目:
汕头市重点科技计划项目 (No:汕府科2010-63-27)

详细信息

通讯作者: 王双乐, E-mail:shuangle999@163.com

中图分类号: R739.63

收稿日期: 2011-12-04

Expressions and correlation of HPA,CK2β and HIF-1α in nasopharyngeal carcinoma

- Department of Otolaryngology Head and Neck Surgery, Shantou City Central Hospital, Shantou, 515031, China

More Information

Corresponding author: WANG Shuangle, E-mail: shuangle999@163.com

Received Date: 04 December 2011

摘要

摘要: 目的:检测乙酰肝素酶(HPA)、蛋白激酶CK2β(CK2β)、缺氧诱导因子-1α(HIF-1α)在鼻咽癌(NPC)组织中的表达及其与临床特征之间的关系;并分析三者在NPC组织中表达的相关性。方法:采用免疫组织化学染色技术Super-Vision法检测49例NPC组织标本及15例慢性鼻咽炎(CNT)组织标本中HPA、CK2β及HIF-1α的表达水平。结果:HPA、CK2β与HIF-1α在NPC组织中的表达水平均高于CNT组织中的表达,差异均有统计学意义(P＜0.05);HPA、CK2β与HIF-1α的表达与NPC临床分期、治疗后有无复发或转移相关(P＜0.05),与患者的性别无关(P＞0.05);HIF-1α的表达水平与NPC患者的年龄密切相关(P＜0.01),而HPA、CK2β的表达水平则与NPC患者的年龄无关(P＞0.05);HPA、CK2β与HIF-1α任意两者间在NPC组织中的表达均呈正相关性(P＜0.05)。结论:HPA、CK2β和HIF-1α参与了NPC的发生和发展、浸润及转移;在NPC的发生发展过程中,HPA、CK2β和HIF-1α之间可能存在协同作用。
- 鼻咽肿瘤 /
- 乙酰肝素酶 /
- 蛋白激酶CK2β /
- 缺氧诱导因子-1α
Abstract: Objective:To investigate the expression of HPA,CK2β and HIF-1α gene in nasopharyngeal carcinoma(NPC) tissues, and the correlation between their expression with the clinical characteristics of NPC and the relativity of HPA,CK2β and HIF-1α gene in NPC tissues.Method:HPA, CK2β and HIF-1α were detected with Super-Vision immunohistochemical method using antibody in 49 NPC specimens and 30 specimens with chronic nasopharyngitis tissue(CNT).Result:The expression of HPA,CK2β and HIF-1α in NPC tissue were significantly higher than those in CNT tissue (P＜0.05,separately). The expression of HPA,CK2β and HIF-1α were significantly related to the TNM stage and whether recurrence or metastasis occur after treatment (P＜0.05,separately), but there was no obvious correlation between its expression and the sex of NPC patient(P>0.05). The expression of HIF-1α was significantly related to the age of NPC patient (P＜0.05), while HPA,CK2β were not. The expression of HPA,CK2β and HIF-1α in NPC tissue was positively correlated with each other (P＜0.05, separately).Conclusion:HPA, CK2β and HIF-1α play synergetic role in development of NPC, which plays an important role in invasiveness,recurrence and metastasis of NPC. There could be a positive cooperation among HPA, CK2β and HIF-1α in the carcinogenesis and development of NPC.
- nasopharyngeal neoplasm /
- heparanase /
- protein kinase CK2β /
- hypoxia inducible factor-1α

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