Comparison of ELISA and Pharmacia UNICAP detection reagent for House dust mite specific IgG4
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摘要: 目的:对屋尘螨特异性IgG4 ELISA检测试剂与法玛西亚UNICAP IgG4检测试剂进行对比研究。方法:首先对屋尘螨特异性IgG4 ELISA检测试剂相关性能指标进行研究。对比ELISA试剂与法玛西亚UNICAP检测试剂的浓度校准曲线。同时采用以上2种检测试剂和方法,对临床上65份行屋尘螨特异性免疫治疗患者的血清标本进行检测,并对检测结果进行相关分析。结果:屋尘螨特异性IgG4 ELISA检测试剂具有良好的性能指标(批内差CV=3.48%,批间差CV=5.78%)。ELISA试剂与法玛西亚UNICAP检测试剂的校准曲线非常相似,在0.003~0.150 μg/ml检测范围内呈现良好的线性关系。对临床上特异性免疫治疗前后65份血清进行检测,ELISA与法玛西亚UNICAP检测结果相关系数为0.93。结论:屋尘螨特异性IgG4 ELISA检测试剂与法玛西亚UNICAP检测试剂具有良好的一致性,可望在临床上广泛开展应用。Abstract: Objective: To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.Method: The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.Result: The stability of ELISA reagent was good with low intra and inter assay variations(3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.Conclusion: The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
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Key words:
- house dust mite /
- allergic rhinitis /
- specific IgG4 /
- ELISA /
- UNICAP
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