血必净对OVA诱导变应性鼻炎小鼠的干预作用及其对HO-1的影响

宋冬梅; 牛英豪; 王建涛; 薛静; 吕欣; 杨建旺; 王宝山

doi:10.13201/j.issn.1001-1781.2013.16.013

血必净对OVA诱导变应性鼻炎小鼠的干预作用及其对HO-1的影响

- 河北医科大学第一医院耳鼻咽喉及变态反应科(石家庄, 050031)

详细信息

通讯作者: 王宝山,E-mail:wbsent@126.com

中图分类号: R765.21

收稿日期: 2012-07-24

Anti-allergic effects of Xuebijing and potential role of heme oxygenase-1against ovalbumin-induced murine allergic rhinitis model

- Department of Otolaryngology Head and Neck Surgery, Devision of Allergic Medicine, First Hospital of Hebei Medical University, Shijiazhuang, 050031, China

More Information

Corresponding author: WANG Baoshan, E-mail: wbsent@126.com

Received Date: 24 July 2012

摘要

摘要: 目的:研究血必净在OVA诱导的小鼠变应性鼻炎（AR）模型中的干预作用及其对血红素加氧酶-1（HO-1）表达的影响,探讨血必净注射液气道局部雾化吸入给药的可行性。方法:建立OVA诱导的小鼠AR模型,用血必净[1ml/（kg·d）]和地塞米松[30mg/（kg·d）]分别进行气道雾化治疗,对照组用PBS（pH 7.4）代替;检测各组小鼠鼻腔灌洗液中嗜酸粒细胞计数;ELISA法检测鼻腔灌洗液中炎性因子、OVA特异性IgE的表达水平;苏木精-伊红染色法检测鼻黏膜及肺组织的形态学变化;用Western Blot、Real-time PCR及免疫组织化学检测鼻黏膜及肺组织中HO-1的表达。结果:经血必净气道局部雾化吸入给药处理的小鼠可明显降低气道中的嗜酸粒细胞、炎性因子IL-4、IL-5、IL-13和TNF-α的表达水平,提高IFN-γ的表达水平;降低鼻腔灌洗液中OVA特异性IgE的表达水平;减少鼻黏膜和肺组织中黏液产生和炎症细胞的浸润;诱导鼻黏膜及肺组织HO-1的表达。结论:血必净注射液气道局部雾化吸入给药可以抑制OVA诱导的小鼠AR模型中炎性因子产生,逆转Th1/Th2失衡,其治疗作用可能与诱导鼻黏膜组织HO-1的高表达有关。
- 鼻炎,变应性 /
- 嗜酸粒细胞 /
- 细胞因子 /
- 血红素加氧酶-1
Abstract: Objective:In this study,we investigated the anti-inflammation effects of Xuebijing in OVA-induced murine allergic rhinitis model.Furthermore,we determined whether heme oxygenase（HO）-1 is required for the protective activity of Xuebijing.Method:Airways of OVA-sensitized mice exposed to OVA challenge developed eosinophilia,mucus hypersecretion and increased cytokine levels.Levels of interleukin IL-4,IL-5,IL-13,and tumor necrosis factor（TNF）-alpha in nasal lavage fluid were measured using enzyme-linked immunosorbent assays（ELISAs）.Lung tissue and nasal mucosa sections were stained with Mayer’s hematoxylin and eosin for assessment of cell infiltration and mucus production,Immunohistochemistry,Real-time PCR and Western Blot analyses for HO-1 protein expression.Result:Orally administered Xuebijing significantly inhibited the number of OVA-induced inflammatory cells and IgE production,along with reduced T-helper（Th）2 cytokine levels,such as IL-4,IL-5 and IL-13,improved the level of IFN-γ,in nasal lavage fluid.In addition,Xuebijing induced a marked decrease in OVA-induced inflammatory cell infiltration and mucus production in nasal and lung tissues.These effects were correlated with HO-1 mRNA and protein induction.Conclusion:Our results indicate that Xuebijing protects against OVA-induced airway inflammation,at least in part,via HO-1 upregulation.
- rhinitis,allergic /
- eosinophilia /
- cytokine /
- HO-1

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参考文献(21)

[1]	AMIN K. The role of mast cells in allergic inflammation[J]. Respir Med,2012, 106:9-14.
[2]	HALIM T Y, KRAUB R H, SUN A C, et al. Lung natural helper cells are a critical source of th2 cell-type cytokines in protease allergen-induced airway inflammation[J]. Immunity, 2012, 36:451-463.
[3]	LEE M Y, SEO C S, HA H, et al. Protective effects of Ulmus davidiana var japonica against OVA-induced murine asthma model via upregulation of heme oxygenase-1[J]. Ethnopharmacol, 2010, 130:61-69.
[4]	SOARES M P, MARGUTI I, CUNHA A, et al. Immunoregulatory effects of HO-1:how does it work[J]. Curr Opin Pharmacol, 2009, 9:482-489.
[5]	GOZZELINO R, JENEY V, SOARES M P. Mechanisms of cell protection by heme oxygenase-1[J]. Annu Rev Pharmacol Toxicol, 2010, 50:323-354.
[6]	TZIMA S, VICTORATOS P, KRANIDIOTI K, et al. Myeloid heme oxygenase-1 regulates innate immunity and autoimmunity by modulating IFN-beta production[J]. Exp Med,2009, 206:1167-1179.
[7]	PARK D J, AGARWAL A, GEORGE J F. Heme oxygenase-1 expression in murine dendritic cell subpopulations[J]. Am J Pathol, 2010,176:28319-28319.
[8]	王念,沈国强.血必净对脑梗死合并慢性阻塞性肺疾患急性发作的治疗作用及影响[J].实用心脑肺血管病杂志, 2010, 18(11):1641-1641.
[9]	郭保军.血必净注射液治疗慢性肺源性心脏病急性加重期的疗效观察[J].实用心脑肺血管病杂志, 2010, 18(3):358-358.
[10]	陈建南,何平,孙定卫.血必净注射液对重症肺炎患者的影响研究[J].中国全科医学, 2010, 13(9):3081-3081.
[11]	瞿秋,杨菊,张燕祺.血必净注射液辅助治疗老年下呼吸道感染的疗效研究[J].实用心脑肺血管病杂志, 2010, 18(10):1416-1416.
[12]	王卫华,安云芳,赵长青.变应性鼻炎对小鼠鼻黏膜核因子-κB p65和糖皮质激素受体表达的影响[J].中国中西医结合耳鼻咽喉科杂志, 2009, 17(1):4-9.
[13]	李春亮,侯亚利.丹参预处理干预心肌缺血再灌注损伤的研究进展[J].河北北方学院学报(医学版),2007,24(5):74-76.
[14]	杨眉.丹参、黄芪注射液的药理作用及临床应用[J].浙江中医药大学学报,2007,31(3):379-379.
[15]	万生芳.当归、红芪超滤膜提取物对急性心肌缺血大鼠NO和CK-MB影响的实验研究[J].中华中医药学刊,2007,25(1):153-155.
[16]	AMEREDES BT, OTTERBEIN LE, KOHUT LK, et al. Low-dose carbon monoxide reduces airway hyperresponsiveness in mice[J]. Am J Physiol Lung Cell Mol Physiol, 2003, 285:1270-1286.
[17]	余少卿,章如新,刘国钧,等.血红素氧合酶-1在变应性鼻炎豚鼠模型的鼻黏膜中的表达[J].临床耳鼻咽喉科杂志,2005,19(11):504-506.
[18]	WEN M C, WEI C H, HU G Q,et al. Efficacy and tolerability of anti-asthma herbal medicine intervention in adult patients with moderate severe allergic asthma[J]. Allergy Clin Immunol,2005,116, 517-524.
[19]	刘延利,孙宏伟.中药雾化吸入去敏方法治疗过敏性鼻炎110例临床观察[J].中国医师杂志,2004,24(S1):242-242.
[20]	李志云.红霉素+地塞米松+中药雾化吸入治疗慢性咽喉炎体会[J].中国社区医师, 2007, 23(2):136-136.
[21]	王秀霞.中药超声雾化吸入治疗老年慢性支气管炎疗效观察[J].中国乡村医药,2007,20(1):52-53.