喉鳞状细胞癌组织中IL-17的表达及其临床意义

王雅堂; 杨见明; 许海艳

doi:10.13201/j.issn.1001-1781.2013.14.004

喉鳞状细胞癌组织中IL-17的表达及其临床意义

- 安徽医科大学第二附属医院耳鼻咽喉科(合肥, 230601)
基金项目:
安徽省科技厅重点资助课题(No:12070403060)

详细信息

通讯作者: 杨见明,E-mail:jmyang88@163.com

中图分类号: R739.65

收稿日期: 2013-02-08

Expression of IL-17 in laryngeal squamous cell carcinoma tissues and the clinical significance

- Department of Otorhinolaryngology, the Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, China

More Information

Corresponding author: YANG Jianming, E-mail: jmyang88@163.com

Received Date: 08 February 2013

摘要

摘要: 目的:检测喉鳞状细胞癌组织中IL-17的表达情况,评价IL-17的表达在喉鳞状细胞癌生物学行为和预后中的价值。方法:采用免疫组化SP法检测71例喉鳞状细胞癌及21例癌旁组织中IL-17的表达。结果:喉鳞状细胞癌组织中IL-17的表达率为54.9%(39/71),与癌旁组织(28.6%)对比差异有统计学意义(P<0.05);IL-17的表达与喉癌的分期、肿瘤分化、区域淋巴结转移、远处转移及复发有关(均P<0.05);Log-rank检验显示患者预后与肿瘤原发灶部位、原发灶分期、区域淋巴结转移、远处转移、IL-17的表达有关(P<0.05),多因素Cox回归分析发现IL-17不是影响患者预后的独立危险因素。结论:IL-17与喉鳞状细胞癌的发生、发展以及预后关系密切,是喉鳞状细胞癌患者的不良预后因素,可为喉鳞状细胞癌的抗肿瘤免疫治疗提供一定的理论依据。
- 喉鳞状细胞癌 /
- IL-17
Abstract: Objective:To investigate the expression of interleukin 17 in laryngeal squamous cell carcinoma tissues,and to evaluate the value of interleukin-17 in the biological behavior and prognosis of laryngeal squamous cell carcinom.Method:Expressions of IL-17 were analyzed in 71 laryngeal squamous cell carcinoma samples and 21 cases of adjacent tissues of carcinoma as control by immunohistochemistry.Result: The expression of IL-17 in laryngeal squamous cell carcinoma tissues was obviously higher than that in adjacent tissues(P< 0.05).The expression of IL-17 was associated with tumor T staging,tumor differentiation,regional lymph node metastasis,distant metastasis and recurrence(P< 0.05).Log-rank test showed that the prognosis of patients was related to the primary tumor site,tumor stage,regional lymph node metastasis,distant metastasis and IL-17 expression(P< 0.05),While Cox multiple factors analysis showed that IL-17 was not an independent prognostic factors.Conclusion: The expression of IL-17 may be the vital index in laryngeal squamous cell carcinoma,and it can provide some theoretical basis for the anti-tumor immunotherapy for laryngeal squamous cell carcinoma.
- laryngeal squamous cell carcinoma /
- IL-17

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参考文献(24)

[1]	BALKWILL F,MANTOVANI A.Inflammation and cancer:back to Virchow[J]?Lancet,2001,357:539-545.
[2]	SCHWARTSBURD P M.Chronic inflammation as induc-tor of pro-cancer microenvironment:pathogenesis of dysregulated feedback control[J].Cancer Metastasis Rev,2003,22:95-102.
[3]	ZHANG B,RONG G H,WEI H F,et al.The prevalence of Th17cells in patients with gastric cancer[J].Bioe-hem Biophys Res Commun,2008,374:533-537.
[4]	LE GOUVELLO S,BASTUJI-GARIN S,ALOULOU N,et al.High prevalence of Foxp3and IL17in MMR-profi-cient colorectal carcinomas[J].Gut,2008,57:772-779.
[5]	ZHANG J P,YAN J,XU J,et al.Increased intratumoral IL-17-Producing cells correlate with poor survival in hepatocellular carcinoma patients[J].J Hepatol,2009,50:980-989.
[6]	MIYAHARA Y,ODUNSI K,CHEN W,et al.Generation and regulation of human CD4+IL-17-producing T cells in ovarian cancer[J].Proc Natl Acad Sci USA,2008,105:15505-15510.
[7]	DHODAPKAR K M,BARHUTO S,MATTHEWS P,et al.Dendritic cells mediate the induction of polyfunc-tional human IL17-Producing cells(Th17-1cells)en-riched in the bone marrow of patients with myeloma[J].Blood,2008,112:2878-2885.
[8]	VELDHOEN M,HOCKING R J,FLAVELL R A,et al.Signals mediated by transforming growth factor-beta ini-tiate autoimmune encephalomyelitis,but chronic in-flammation is needed to sustain disease[J].Nat Immu-nol,2006,7:1151-1156.
[9]	DONG C.Diversification of T-helper-cell lineages:find-ing the family root of IL-17-producing cells[J].Nat Rev Immunol,2006,6:329-333.
[10]	YE P,RODRIGUEZ F H,KANALY S,et al.Require-ment of interleukin17receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor ex-pression,neutrophil recruitment,and host defense[J].J Exp Med,2001,194:519-527.
[11]	OUYANG W,KOLLS J K,ZHENG Y.The biological functions of T helper17cell effector cytokines in in-flammation[J].Immunity,2008,28:454-467.
[12]	SUN Y C,ZHOU Q T,YAO W Z.Sputum interleukin-17is increased and associated with airway neutrophilia in patients with severe asthma[J].Chin Med J,2005,118:953-956.
[13]	DONG G,YE R,SHI W,et al.IL-17induces autoanti-body overproduction and peripheral blood mononuclear cell overexpression of IL-6in lupus nephritis patients[J].Chin Med J,2003,116:543-548.
[14]	施沛青,朱书,钱友存.IL-17的信号传导及功能研究[J].中国细胞生物学学报,2011,33(4):345-357.
[15]	NUMASAKI M,FUKUSHI J,ONO M,et al.Interleu-kin-17promotes angiogenesis and tumor growth[J].Blood,2003,101:2620-2627.
[16]	NUMASAKI M,WATANABE M,SUZUKI T,et al.IL-17enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogene-sis[J].J Immunol,2005,175:6177-6189.
[17]	HORLOCK C,STOTT B,DYSON P J,et al.The effects of trastuzumab on the CD4+CD25+FoxP3+andCD4+IL17A+T-cell axis in patients with breast caneer[J].Br J Cancer,2009,100:1061-1067.
[18]	李琳,朱冬冬,董震.喉癌组织炎症细胞因子和受体相关基因的表达谱[J].中国耳鼻咽喉头颈外科,2008,15(2),67-70.
[19]	MENG C D,ZHU D D,LI L,et al.Overexpression of interleukin-17in tumor-associated macrophages is cor-related with the differentiation and angiogenesis of la-ryngeal quamous cell carcinoma[J].Chinese Medical J,2012,125:1603-1607.
[20]	KOBAYASHI N,HIRAOKA N,YAMAGAMI W,et al.FOXP3+regulatory T cells affect the development and progression of hepatocarcinogenesis[J].Clin Cancer Res,2007,13:902-911.
[21]	SFANOS K S,BRUNO T C,MARIS C H,et al.Pheno-typic analysis of pros-tate-infiltrating lymphocytes re-veals TH17and Treg skewing[J].Clin Cancer Res,2008,14:3254-3261.
[22]	MARUYAMA T,KONO K,MIZUKAMI Y,et al.Distri-bution of Th17cells and FoxP3(+)regulatory T cells in tumor-infiltrating lymphocytes,tumor-draining lymph nodes and peripheral blood lymphocytes in patients with gastric cancer[J].Cancer Science,2010,101:1947-1954.
[23]	夏良平,曾宗渊,魏茂文,等.影响喉癌生存率的单因素和多因素分析[J].中国肿瘤,2002,11(11):655-657.
[24]	董文汇,郭启云.喉癌99例临床分析[J].中华现代眼耳鼻喉科杂志,2008,5(2):123-125.