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Abstract: So far, there is no effective treatment for most of patients with sensorineural hearing loss.Many studies have shown that gene therapy has become more promising in terms of protecting the inner ear from these diseases.In recent years, inner ear gene therapy has become a research focus in the field of otology.It has not yet appeared a feasible technique for inner ear gene therapy in clinical application due to the particularity of the structure and function of inner ear.On the other hand, in order to avoid the adverse effects of carcinogenic and immunity caused by viral vectors, many scholars devote themselves to develop new gene vectors based on non-viral materials, such as liposomes, polymers and other nanomaterials.In recent years, many great progresses have been made in development and application of non-viral vectors, which showing an inspiring prospect.The research status, progress and prospects of non-viral vectors in inner ear gene therapy will be discussed in this review.
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Key words:
- non-viral vector /
- gene vector /
- inner ear /
- gene therapy
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[1] 孙虹,蒋明,朱晒红. 用羟基磷灰石纳米粒作内耳基因治疗新载体的体内外研究[J]. 中华耳鼻咽喉头颈外科杂志, 2008, 43(1):51-57.
[2] SUN H, HUANG A, CAO S. Current status and prospects of gene therapy for the inner ear[J]. Hum Gene Ther, 2011, 22:1311-1322.
[3] SERIKAWA T, KIKUCHI A, SUGAYA S, et al. In vitro and in vivo evaluation of novel cationic liposomes utilized for cancer gene therapy[J].J Control Release,2006,113:255-260.
[4] WAREING M, MHATRE A N, PETTIS R, et al. Cationic liposome mediated transgene expression in the guinea pig cochlea[J]. Hear Res,1999,128:61-69.
[5] 时利,董明敏,时文杰,等. 碱性成纤维细胞生长因子真核表达载体的构建及其在内耳基因治疗中的实验研究[J].中华耳鼻咽喉头颈外科杂志,2003,38(1):21-23.
[6] 邓志宏, 王锦玲, 邱建华, 等. 神经营养因子3基因转染对庆大霉素性耳聋保护作用的实验研究[J].临床耳鼻咽喉科杂志,2004,18(4):231-233.
[7] 蒋明,张永全,贺广湘,等. 阳离子脂质体介导小鼠原代螺旋神经节细胞NT-3基因转染的实验研究[J].中国耳鼻咽喉颅底外科杂志, 2006,12(1):10-13.
[8] MAEDA Y, FUKUSHIMA K, KAWASAKI A, et al. Cochlear expression of a dominant-negative GJB2R75W construct delivered through the round window membrane in mice[J]. Neurosci Res,2007,58:250-254.
[9] ZOU J,SOOD R,RANJAN S,et al. Manufacturing and in vivo inner ear visualization of MRI traceable liposome nanoparticles encapsulating gadolinium[J].J Nanobiotechnology,2010,8:32-32.
[10] 胡云霞,原续波,张晓金,等. 阳离子多聚物纳米基因载体系统的研究进展[J].北京生物医学工程,2003,22(4):299-302.
[11] WANG H, SHI H B, YIN S K. Polyamidoamine dendrimers as gene delivery carriers in the inner ear:How to improve transfection efficiency[J].Exp Ther Med, 2011,2:777-781.
[12] NOUSHI F, RICHARDSON R T, HARDMAN J, et al. Delivery of neurotrophin-3 to the cochlea using alginate beads[J].Otol Neurotol,2005,26:528-533.
[13] TAN B T, FOONG K H, LEE M M,et al. Polyethylenimine-mediated cochlear gene transfer in guinea pigs[J].Arch Otolaryngol Head Neck Surg, 2008, 134:884-891.
[14] PRAETORIUS M,PFANNENSTIEL S,KLINGMANN C, et al. Expression patterns of non-viral transfection with GFP in the organ of Corti in vitro and in vivo. Gene therapy of the inner ear with non-viral vectors[J].HNO,2008,56:524-529.
[15] BUCKIOVA D, RANJAN S, NEWMAN T A, et al. Minimally invasive drug delivery to the cochlea through application of nanoparticles to the round window membrane[J].Nanomedicine (Lond),2012,7:1339-1354.
[16] TAMURA T, KITA T, NAKAGAWA T, et al.Drug delivery to the cochlea using PLGA nanoparticles[J].Laryngoscope,2005, 115:2000-2005.
[17] KOPKE R D,WASSEL R A,MONDALEK F,et al.Magnetic nanoparticles:inner ear targeted molecule delivery and middle ear implant[J].Audiol Neurootol,2006,11:123-133.
[18] PRAETORIUS M, BRUNNER C, LEHNERT B, et al. Transsynaptic delivery of nanoparticles to the central auditory nervous system[J].Acta Otolaryngol,2007,127:486-490.
[19] 丁大连,蒋涛,亓卫东,等.内耳科学[M].北京:中国科学技术出版社,2010:123-125.
[20] GE X, JACKSON R L, LIU J, et al. Distribution of PLGA nanoparticles in chinchilla cochleae[J]. Otolaryngol Head Neck Surg, 2007, 137:619-623.
[21] ZHU S H,HUANG B Y,ZHOU K C,et al. Hydroxyapatite Nanoparticles as a Novel Gene Carrier[J].J Nanoparticle Res,2004,6:307-311.
[22] 蒋明,张永全,贺广湘,等. 羟基磷灰石纳米颗粒介导NT-3基因对豚鼠兴奋毒性损伤耳蜗的保护作用[J].中南大学学报(医学版), 2007,32(4):563-567.
[23] FANT K,ESBJORNER E K,JENKINS A,et al. Effects of PEGylation and acetylation of PAMAM dendrimers on DNA binding, cytotoxicity and in vitro transfection efficiency[J].Mol Pharm,2010,7:1734-1746.
[24] REN L L, WU Y,HAN D,et al. Math1 gene transfer based on the delivery system of quaternized chitosan/Na-carboxymethyl-beta-cyclodextrin nanoparticles[J]. J Nanosci Nanotechnol, 2010, 10:7262-7265.
[25] 吴南, 陈志婷, 李敏, 等. 基于聚酰胺的非病毒载体携带Math1基因进行体外基因转导的有效性和安全性评价[J].中华耳科学杂志, 2011,9(3):258-263.
[26] ROY S,JOHNSTON A H,NEWMAN T A,et al. Cell-specific targeting in the mouse inner ear using nanoparticles conjugated with a neurotrophin-derived peptide ligand:potential tool for drug delivery[J].Int J Pharm,2010,390:214-224.
[27] WU X,DING D,JIANG H,et al. Transfection using hydroxyapatite nanoparticles in the inner ear via an intact round window membrane in chinchilla[J].J Nanoparticle Res,2012,14:1-13.
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