亚砷酸联合顺铂对人鼻咽癌裸鼠移植瘤的抑制作用及对DAPK的影响

黄雪琴; 王晓刚; 胡俊丽; 周慧; 周克元; 张月飞

doi:10.13201/j.issn.1001-1781.2013.09.008

亚砷酸联合顺铂对人鼻咽癌裸鼠移植瘤的抑制作用及对DAPK的影响

- 1 广东医学院(广东湛江, 524023);
- 2 广东医学院附属医院耳鼻咽喉科
基金项目:
广东省科技计划项目

湛江市科技攻关计划项目(No:2011C3109010)

广东医学院青年基金(No:Q2010020)

详细信息

通讯作者: 张月飞,E-mail:hxqcong@163.com

中图分类号: R739.6

收稿日期: 2013-01-21

Inhibitory effect of arsenic trioxide combined with cisplatin on human nasopharyngeal carcinoma xenograft and DAPK in nude mice

- 1 Guangdong Medical College, Zhanjiang, 524023, China;
- 2 Department of Otolaryngology, Affiliated Hospital of Guangdong Medical College

More Information

Corresponding author: ZHANG Yuefei, E-mail: hxqcong@163.com

Received Date: 21 January 2013

摘要

摘要: 目的:研究亚砷酸(As₂O₃)联合顺铂(DDP)对人鼻咽癌CNE-2Z裸鼠移植瘤的生长抑制作用及对死亡相关蛋白激酶(DAPK)的影响。方法:建立人鼻咽癌裸鼠移植瘤模型,随机分为对照组、As₂O₃组、DDP组和As₂O₃加DDP组,比较各组的抑瘤作用;并对标本分别进行光镜、原位末端标记(TUNEL)检测,采用实时荧光定量PCR法与免疫组织化学方法检测DAPK的表达。结果:As₂O₃和As₂O₃加DDP均能显著抑制人鼻咽癌裸鼠移植瘤的生长,诱导肿瘤细胞凋亡,并能上调DAPK的表达。结论:As₂O₃可抑制人鼻咽癌CNE-2Z裸鼠移植瘤的生长,该作用与诱导人鼻咽癌CNE-2Z裸鼠移植瘤细胞凋亡、上调DAPK的表达相关。As₂O₃与DDP联用可起协同作用。
- 亚砷酸 /
- 鼻咽癌 /
- 死亡相关蛋白激酶 /
- 凋亡 /
- 裸鼠
Abstract: Objective: To study the inhibitory effect of Arsenic Trioxide(As₂O₃) combined with diamminedichloroplatinum(DDP) on the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, and to explore the possible effect mechanisms of the antitumor.Method: The models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into four groups, control group, As₂O₃ group, DDP group and As₂O₃+DDP group. The effect of antitumor on each group was studied. The specimen obtained from the mice were detected by opticalmicroscope and tdt-mediated dutp rock end labeling(tunel) method . Expression of DAPK was detected by real time-PCR and immunohistochemistry.Result: As₂O₃ group and As₂O₃+DDP group could obviously inhibit the growth of tumor, induce the apoptosis of human nasopharyngeal carcinoma cell and up-regulate the expression of RASSF1A.Conclusion: As₂O₃ can greatly inhibit the growth of human nasopharyngeal carcinoma cell strain CNE-2Z xenograft in nude mice, which were related to the induced apoptosis of human nasopharyngeal carcinoma cell and up-regulated expression of DAPK Combination of As₂O₃ with DDP seem to be more effective.
- arsenic trioxide /
- nasopharyngeal carcinoma /
- death-associated protein kinase /
- apoptosis /
- nude mice

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