The role of sinomenine in treatment of allergic rhinitis mice model and its mechanism
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摘要: 目的:研究青藤碱对变应性鼻炎的治疗作用并初步探究其相关机制。方法:用卵清蛋白(OVA)致敏BALB/c小鼠制作变应性鼻炎小鼠模型,对照组用生理盐水替代,青藤碱治疗组于鼻部激发时开始换用含青藤碱的饲料喂养。末次激发24h后,各组小鼠取鼻部组织固定,苏木精-伊红染色;ELISA检测血清中特异性抗OVA-IgE、IL-4和IFN-γ浓度;Western Blot检测转录因子T-bet和GATA3表达水平。结果:青藤碱治疗组的小鼠鼻黏膜无明显腺体增生及嗜酸粒细胞浸润。血清中特异性抗OVA-IgE、IL-4和IFN-γ浓度,T-bet和GATA3表达水平均较变应性鼻炎组降低。结论:青藤碱对变应性鼻炎小鼠模型具有一定的治疗作用,其机制可能是通过改善Th1/Th2细胞失衡发挥作用。Abstract: Objective:To explore the role of sinomenine in treatment of allergic rhinitis mice model and its possible mechanism.Method:We used ovalbumin(OVA) to make allergic rhinitis model of BALB/c mice.Saline was used in the control group.When we challenged the mice with OVA intranaslly,the mice in sinomenine treatment group were feed by the food containing sinomenine.Mice were then killed 24 h after the last OVA challenge.The noses of mice from each group were removed en bloc and fixed,then each section was stained with hematoxylin and eosin.ELISA assay was used to measure the concentration of anti-OVA IgE,IL-4 and IFN-γ.The proteins expressive level of T-bet and GATA3 were examined.Result:Nasal mucosa of the mice in sinomenine treatment group were not hyperplasia and without obvious infiltration of eosinophils.The concentration of anti-OVA IgE,IL-4 and IFN-γ in the serum and T-bet and GATA3 expression levels of sinomenine treatment group were lower than those of allergic rhinitis group.Conclusion:The sinomenine can be used to treat allergic rhinitis mice,and the mechanism may rely on the improvements of the Th1/Th2 imbalance.
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Key words:
- rhinitis /
- allergic /
- BALB/c mice /
- sinomenine /
- Th1/Th2 cells
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