神经干细胞移植到神经导管修复大鼠面神经缺损的研究

施勇; 周梁; 田洁; 汪洋

doi:10.13201/j.issn.1001-1781.2012.22.003

神经干细胞移植到神经导管修复大鼠面神经缺损的研究

- 复旦大学附属眼耳鼻喉科医院耳鼻咽喉科(上海, 200031)
基金项目:
上海市卫生局青年基金项目(No:2009y013)

详细信息

通讯作者: 周梁,E-mail:zhoulent@126.com

中图分类号: R745.1

收稿日期: 2012-04-09

Transplanting neural stem cells in nerve conduit to promote rats facial nerve regeneration

- Department of Otolaryngology, Eye and ENT Hospital, Fudan University, Shanghai, 200031, China

More Information

Corresponding author: ZHOU Liang, E-mail: zhoulent@126.com

Received Date: 09 April 2012

摘要

摘要: 目的:观察移植神经干细胞到可降解的聚乳酸/聚羟基乙酸(PLGA)神经导管修复大鼠面神经缺损的可行性。方法:20只SD大鼠随机分为2组,每组进行右侧面神经损伤手术,分别移植PLGA导管(A组)或含有神经干细胞的PLGA管(B组)。术后4、12周分别通过电生理检测、轴突形态学检测等方法来评估面神经的修复效果。结果:术后4周和12周,B组在神经动作电位的振幅分别为(27.53±7.48)、(141.23±21.18)μV,轴突的面积为(10 539.48±3 605.35)、(30 076.22±7 614.05)μm²,都高于A组[术后4周和12周,动作电位振幅为(13.03±11.38)、(118.50±22.70)μV; 轴突面积为(6 633.20±3 759.30)、(20 772.53±10 903.65)μm²](P<0.05)。结论:神经干细胞移植可能会促进面神经的再生。
- 神经再生 /
- 移植 /
- 干细胞 /
- 神经导管
Abstract: Objective: To construct a biodegradable PLGA nerve conduit (NC) filled with NSCs in order to improve facial nerve regeneration. Method: SD rats were subjected to right facial nerve transection, and PLGA NCs filled with NSCs were used to bridge the nerve gap. Facial nerve regeneration was assessed 4 and 12 weeks after surgery, through electrophysiological testing, and morphometric analysis of axons. Result: Nerve action potential amplitude, and axonal area were significantly greater in the NSCs group than the empty NC group. Conclusion: NSCs transplantation may improve regeneration of the facial nerve.
- nerve regeneration /
- transplantation /
- stem cells /
- nerve conduit

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