Detection and correlation of lipopolysaccharide,vitamin D receptor and matrix metalloproteinase-9 in the middle ear cholesteatoma
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摘要: 目的:探讨脂多糖(LPS)、基质金属蛋白酶-9(MMP-9)在中耳胆脂瘤中的含量及表达情况和它们之间的关系。方法:取29例胆脂瘤型中耳炎患者(胆脂瘤组),其中18例来自这些胆脂瘤患者的正常外耳道皮肤,作为对照组。一部分采用鲎变性细胞溶解物测定法(LAL法)作LPS含量测定;另一部分经甲醛固定、石蜡包埋,用作鼠抗人MMP-9蛋白的免疫组织化学测定。结果:LPS在胆脂瘤组中的含量比对照组高(均为取对数后数值):胆脂瘤组M=0.7390,IQR=0.6203;对照组M=-0.2538,IQR=1.1692(P<0.01)。在两种类型的胆脂瘤组织中,广泛型胆脂瘤组M=0.8403,IQR=0.5254;局限型胆脂瘤组M=0.4048,IQR=0.6139,广泛型的LPS含量比局限型高(P<0.05)。MMP-9蛋白阳性定位于细胞质,呈棕黄色颗粒状。在胆脂瘤标本中,MMP-9蛋白阳性率为79.3%,与外耳道皮肤相比具有显著差异(P<0.05);经Spearman 检验LPS含量与MMP-9之间的表达无明显相关性。结论:LPS与胆脂瘤的形成及其骨质破坏有关;MMP-9与胆脂瘤的发生有关;LPS、MMP-9在中耳胆脂瘤发生中的作用尚无明确的联系。Abstract: Objective: To investigate the expression and concentration of lipopolysaccharide(LPS) and matrix metalloproteinase-9(MMP-9) in middle ear cholesteatoma and discuss their relations.Method: Twenty-nine cases of middle ear cholesteatoma tissue, 18 cases of external auditory canal tissue were detected by limulus amebocyte lysate assay (LAL-assay), and expression of MMP-9 protein in formalin-fixed, paraffin-embedded tissues was detected by immunohistochemical method.Result: The concentrations of LPS in cholesteatoma were higher than that in external auditory canal tissues. In group of cholesteatoma: M=0.739 0,IQR=0.620 3, and in group of external auditory canal tissues: M=-0.253 8,IQR=1.169 2(P<0.01). In cholesteatoma groups, in extensive type: M=0.840 3,IQR=0.525 4; in localized type: M=0.404 8,IQR=0.613 9,the concentrations of LPS were higher in extensive cholesteatoma in comparison with localized cholesteatoma(P<0.05). In cholesteatoma epithelium samples, MMP-9 were 79.3%.Compared with external auditory canal epithelium, the expression of MMP-9 was higher in middle ear cholesteatoma epithelium(P<0.05). There was no significant difference in the expression of MMP-9 between two types of cholesteatoma epithelium(P>0.05). LPS, MMP-9 weren't significantly correlated by Spearman test.Conclusion: LPS was responsible for middle ear cholesteatoma and its related bone erosion. MMP-9 was related to the development of middle ear cholesteatoma. There's no correlation between LPS and MMP-9.
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