EGCG对鼻咽癌细胞株CNE-2及相关基因表达的影响

雷迅; 孔中雨; 范才文; 易世江; 向秋; 刘强和

doi:10.13201/j.issn.1001-1781.2012.15.006

EGCG对鼻咽癌细胞株CNE-2及相关基因表达的影响

- 1 桂林医学院附属医院耳鼻咽喉-头颈外科(广西桂林, 541001);
- 2 桂林医学院科学实验中心
基金项目:
广西科学技术厅科研基金资助课题(No:桂科自0640187)

广西卫生厅科研基金资助课题(No:重200652)

详细信息

通讯作者: 孔中雨,E-mail:kongzhongyulql@163.com

中图分类号: R739.63

收稿日期: 2011-08-21

Effects of EGCG on the nasopharyngeal carcinoma cell line CNE-2 and the expression of related gene

- 1 Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Guilin Medical College, Guilin, 541001, China;
- 2 Center Laboratory of Guilin Medical College

More Information

Corresponding author: KONG Zhongyu, E-mail: kongzhongyulql@163.com

Received Date: 21 August 2011

摘要

摘要: 目的:研究EGCG对鼻咽癌细胞系CNE-2的增殖与凋亡作用,探讨其对细胞增殖与凋亡相关基因Bcl-2、Bax、Caspase-3表达的影响。方法:应用MTT实验、流式细胞仪检测细胞的增殖与周期;Hoechst33258荧光染色法检测细胞凋亡;RT-PCR检测Bcl-2、Bax、Caspase-3表达。结果:EGCG明显抑制CNE-2细胞的增殖,诱导其凋亡,该作用具有剂量-效应关系。EGCG抑制CNE-2细胞Bcl-2表达,诱导CNE-2细胞Bax、Caspase-3表达。结论:EGCG在体外具有抗鼻咽癌细胞的作用,此作用可能是通过调节细胞增殖与凋亡基因的表达实现的。
- 表没食子儿茶素没食子酸酯 /
- 鼻咽肿瘤 /
- CNE-2
Abstract: Objective: To study the effects of epigallocatechin-3-gallate(EGCG) on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cell line and analyze the expression of Bcl-2,Bax and Caspase-3 in the cell line which treated with EGCG.Method: MTT assay and flow cytometry were used to analyze cell proliferation and cell cycle. Hoechst33258 fluorescence staining was adopted to study cell apoptosis.RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3.Conclusion: EGCG could significantly inhibit proliferation of CNE-2 cell line and induce its apoptosi with dose-independent relationship.EGCG could suppress the expression of Bcl-2 and induce expression of Bax, Caspase-3.Conclusion: EGCG in vitro has efficacy of anti-nasopharyngeal carcinoma cells, which may be through regulating the expression of cell proliferation and apoptosis genes involved.
- epigallocatechin-3-gallate /
- nasopharyngeal neoplasms /
- CNE-2

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