Jab1和p27<sup>kip1</sup>基因在喉鳞状细胞癌组织和喉癌Hep-2细胞中的表达及意义

李发萍; 曹华; 吴玉瑛

doi:10.13201/j.issn.1001-1781.2012.06.003

Jab1和p27^kip1基因在喉鳞状细胞癌组织和喉癌Hep-2细胞中的表达及意义

- 1 广东省人民医院(广东省医学科学院)耳鼻咽喉科(广州, 510080);
- 2 郑州大学第一附属医院耳鼻咽喉科

详细信息

通讯作者: 曹华,E-mail:caohua1105@yahoo.com.cn

中图分类号: R739.6

收稿日期: 2011-02-13

Expression and their significance of Jab1 p27^kip1 in laryngeal squamous cell carcinoma and Hep-2 cells

- 1 Department of Otolaryngology, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China;
- 2 Department of Otolaryngology, the First Affiliated Hospital, Zhengzhou University

More Information

Corresponding author: CAO Hua, E-mail: caohua1105@yahoo.com.cn

Received Date: 13 February 2011

摘要

摘要: 目的:探讨Jab1和p27^kip1基因在喉鳞状细胞癌组织和喉癌Hep-2细胞中的表达及意义。方法:采用免疫组织化学方法检测50例喉鳞状细胞癌组织和10例癌旁正常组织中Jab1和p27^kip1的表达。采用人工合成Jab1 siRNAⅡ用脂质体2000包裹后转染Hep-2细胞,用RT-PCR方法分析转染后喉癌细胞中Jab1和p27^kip1基因的表达情况。结果:Jab1和p27^kip1蛋白的阳性表达定位为细胞核中,Jab1在人喉鳞状细胞癌组织中高表达,正常喉黏膜中不表达或弱表达;p27^kip1在人喉癌组织中弱表达,正常喉黏膜中强表达;两者呈负相关。Jab1 siRNAⅡ可降低Jab1 mRNA表达,并且随着时间的延长,Jab1 mRNAⅡ在Hep-2细胞中的表达逐渐减弱,而p27^kip1 mRNA的表达则无明显变化。结论:Jab1在人喉鳞状细胞癌组织中表达增强,正常喉黏膜中不表达或弱表达;p27^kip1则正好相反。两者在喉癌中的表达呈负相关。Jab1 siRNAⅡ可以特异性降低Jab1 mRNA在喉癌Hep-2细胞中的表达,为进一步研究Jab1基因在喉癌中的作用奠定了基础。
- 喉鳞状细胞癌 /
- Jab1基因 /
- p27^kip1基因 /
- RNA干扰 /
- Hep-2细胞 /
- RT-PCR
Abstract: Objective: To discuss the expression and the significance of Jab1 p27^kip1 in laryngeal squamous cell carcinoma and Hep-2 cells.Method: Immunohistochemical method was used to examine the expressions of Jab1 and p27^kip1 proteins in 50 cases laryngeal squamous cell carcinomaes and 10 cases normal laryngeal tissues adjacent to laryngeal squamous cell carcinoma. Hep-2 cells were transfected with synthetic Jab1 siRNA by Lipofectamrne2000. RT-PCR method was adopted to examine the mRNA expression of the Jab1 and p27^kip1 gene in Hep-2 cells which was treated with Jab1 siRNAⅡ.Result: Clearly brown staining restricted to nucleus was considered as positive expression of Jab1 and p27^kip1 protein. The expression rate of Jab1 protein in laryngeal squamous cell carcinoma was significantly higher than that of normal laryngeal mucosa, and the expression rate of protein p27^kip1in laryngeal squamous cell carcinoma was significantly lower than that of normal laryngeal mucosa.There was a negative relationship between Jab1 and p27^kip1 protein in laryngeal squamous cell carcinoma. The expression of Jab1 mRNA was suppressed markedly after transfected by Jab1 siRNAⅡ.As the reaction time increased,the expression of Jab1 mRNA of Hep-2 cells decresed significantly,and the expression of p27^kip1 mRNA remained unchanged.Conclusion: The expression rate of Jab1 protein in laryngeal squamous cell carcinoma is significantly higher than that of normal laryngeal mucosa. There is a negative relationship between Jab1 and p27^kip1 protein in laryngeal squamous cell carcinoma. After transfected by Jab1 siRNAⅡin the Hep-2 cells, the expression of Jab1 mRNA is suppressed markedly. Jab1 siRNA would be a good methdology for the further study.
- laryngeal squamous cell carcinoma /
- Jab1 gene /
- p27^kip1 gene /
- RNA interference /
- Hep-2 cell /
- RT-PCR

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